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Literature summary for 1.5.1.32 extracted from
- Vomilenine reductase - a novel enzyme catalyzing a crucial step in the biosynthesis of the therapeutically applied antiarrhythmic alkaloid ojmaline (2002), Bioorg. Med. Chem., 10, 1913-1928.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 2 mM, 45% inhibition, complete inhibition after preincubation with 1 mM EDTA for 30 min, almost the total activity can be restored with 2 mM Co2+, Ni2+ and Mn2+ | Rauvolfia serpentina |  |
| p-hydroxymercuribenzoate | 0.2 mM, complete inhibition | Rauvolfia serpentina | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | most probably a metalloprotein, complete inhibition after preincubation with 1 mM EDTA for 30 min, can be restored with 2 mM Co2+, Ni2+ and Mn2+ | Rauvolfia serpentina |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
gel filtration | Rauvolfia serpentina |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| vomilenine + NADPH | Rauvolfia serpentina | enzyme catalyzes a late step in the biosynthesis of the monoterpenoid indole alkaloid ajmaline | 2-beta-(R)-1,2-dihydrovomilenine + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rauvolfia serpentina | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, Source 30Q, Source 15Phe, 2',5'-ADP-Sepharose | Rauvolfia serpentina |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell suspension culture | - |
Rauvolfia serpentina | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0031 | - |
- |
Rauvolfia serpentina |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| vomilenine + NADPH | - |
Rauvolfia serpentina | 2-beta-(R)-1,2-dihydrovomilenine + NADP+ | non-natural 2alpha-(S)-1,2-dihydrovomilenine is never observed | ? | |
| vomilenine + NADPH | enzyme catalyzes a late step in the biosynthesis of the monoterpenoid indole alkaloid ajmaline | Rauvolfia serpentina | 2-beta-(R)-1,2-dihydrovomilenine + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 43000, SDS-PAGE | Rauvolfia serpentina |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
50% activity at 45°C | Rauvolfia serpentina |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
in phosphate buffer, 50% activity at pH 5.2 and pH 6.8 respectively | Rauvolfia serpentina |
| 6.5 | - |
in phosphate/citric acid buffer | Rauvolfia serpentina |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | 6.8 | - |
Rauvolfia serpentina |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Rauvolfia serpentina | |
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Release 2023.1 (January 2023)
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