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Literature summary for 1.5.1.30 extracted from

  • Vorontsov, I.I.; Minasov, G.; Brunzelle, J.S.; Shuvalova, L.; Kiryukhina, O.; Collart, F.R.; Anderson, W.F.
    Crystal structure of an apo form of Shigella flexneri ArsH protein with an NADPH-dependent FMN reductase activity (2007), Protein Sci., 16, 2483-2490.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Shigella flexneri

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapour diffusion method, using 0.2 M calcium chloride and 20% (w/v) polyethylene glycol 3350 Shigella flexneri

Inhibitors

Inhibitors Comment Organism Structure
Amaranth the presence of 50 mM amaranth reduces the NADPH oxidation rate 2.6fold Shigella flexneri
Ponceau BS the presence of 50 mM Ponceau BS reduces the NADPH oxidation rate 3.7fold Shigella flexneri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28400
-
4 * 28400, dynamic light scattering, ArsH in solution at room temperature does exist predominantly in the tetrameric form Shigella flexneri
113600
-
dynamic light scattering Shigella flexneri

Organism

Organism UniProt Comment Textmining
Shigella flexneri
-
2a strain 2457T
-

Purification (Commentary)

Purification (Comment) Organism
-
Shigella flexneri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
FMN + NADPH
-
Shigella flexneri FMNH2 + NADP+
-
r
additional information no catalytic activity is detected with NADH Shigella flexneri ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 28400, dynamic light scattering, ArsH in solution at room temperature does exist predominantly in the tetrameric form Shigella flexneri
tetramer dimer of dimer, X-ray crystallography Shigella flexneri

Synonyms

Synonyms Comment Organism
ArsH
-
Shigella flexneri
NADPH-dependant FMN reductase
-
Shigella flexneri

Cofactor

Cofactor Comment Organism Structure
FMN
-
Shigella flexneri
NADPH
-
Shigella flexneri