Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Vibrio harveyi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
FMN | native enzyme, at 23°C in 50 mM phosphate buffer, pH 7.0 | Vibrio harveyi | |
0.02 | - |
NADPH | native enzyme, at 23°C in 50 mM phosphate buffer, pH 7.0 | Vibrio harveyi | |
0.072 | - |
FMN | enzyme fused to luciferase, at 23°C in 50 mM phosphate buffer, pH 7.0 | Vibrio harveyi | |
0.31 | - |
NADPH | enzyme fused to luciferase, at 23°C in 50 mM phosphate buffer, pH 7.0 | Vibrio harveyi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26300 | - |
2 * 26300, gel filtration | Vibrio harveyi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio harveyi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
the flavin reductase-luciferase fusion protein is purified using DEAE-cellulose DE-52 column chromatography, DEAE-Sepharose column chromatography, and Superdex 200 gel filtration | Vibrio harveyi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
FMN + NADPH | - |
Vibrio harveyi | FMNH2 + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 26300, gel filtration | Vibrio harveyi |
Synonyms | Comment | Organism |
---|---|---|
FRP | - |
Vibrio harveyi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11.7 | - |
FMN | enzyme fused to luciferase, at 23°C in 50 mM phosphate buffer, pH 7.0 | Vibrio harveyi | |
37.5 | - |
FMN | native enzyme, at 23°C in 50 mM phosphate buffer, pH 7.0 | Vibrio harveyi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Vibrio harveyi | |
NADPH | - |
Vibrio harveyi |