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Literature summary for 1.5.1.30 extracted from

  • Deller, S.; Sollner, S.; Trenker-El-Toukhy, R.; Jelesarov, I.; Guebitz, G.M.; Macheroux, P.
    Characterization of a thermostable NADPH:FMN oxidoreductase from the mesophilic bacterium Bacillus subtilis (2006), Biochemistry, 45, 7083-7091.
    View publication on PubMed
Search for more literature for 1.5.1.30

Inhibitors

Inhibitors Comment Organism Structure
Cibacron Marine azo dye, competitive to NADPH and FMN Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 FMN pH 7.5, 37°C Bacillus subtilis
0.55
-
 NADPH pH 7.5, 37°C Bacillus subtilis
0.65
-
 NADH pH 7.5, 37°C Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
 4 * 21000, SDS-PAGE Bacillus subtilis
76000
-
 gel filtration Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
 enzyme additionally has azoreductase activity
-

Reaction

Reaction Comment Organism Reaction ID
reduced riboflavin + NADP+ = riboflavin + NADPH + H+ bi-bi ternary mechanism, i.e. both FMN and NADPH must bind to the active site before catalysis can occur Bacillus subtilis
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
FMN + NADH preference for NADPH over NADH, rate of reduction is 80 times faster with NADPH Bacillus subtilis FMNH2 + NADP+
-
 ?
FMN + NADPH preference for NADPH over NADH, rate of reduction is 80 times faster with NADPH Bacillus subtilis FMNH2 + NADP+
-
 ?
additional information enzyme additionally has azoreductase activity cleaving the -N=N- bond in azo dyes Bacillus subtilis ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 21000, SDS-PAGE Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
86.5
-
 melting point of protein Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.7
-
 Cibacron Marine pH 7.5, 37°C Bacillus subtilis
60
-
 FMN pH 7.5, 37°C Bacillus subtilis
70
-
 NADPH pH 7.5, 37°C Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
FMN noncovalently bound Bacillus subtilis
NADH
-
 Bacillus subtilis
NADPH
-
 Bacillus subtilis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.003
-
 Cibacron Marine substrate FMN, pH 7.5, 37°C Bacillus subtilis
0.015
-
 Cibacron Marine substrate NADPH, pH 7.5, 37°C Bacillus subtilis