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Literature summary for 1.5.1.30 extracted from
- Activity coupling of Vibrio harveyi luciferase and flavin reductase (FRP): oxygen as a probe (2006), Arch. Biochem. Biophys., 454, 26-31.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km-value of both NADPH and FMN gradually decreases at increasing concentrations of oxygen, with 0.000104 and 0.000019 mM as the upper limits for Km of FMN and NADPH, resp. | Vibrio harveyi | |
| 0.000019 | - |
NADPH | presence of 1.05 mM O2 | Vibrio harveyi |  |
| 0.00013 | - |
FMN | presence of 1.05 mM O2 | Vibrio harveyi | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio harveyi | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| reduced riboflavin + NADP+ = riboflavin + NADPH + H+ | FRP:luciferase coupled reaction can utilize reduced flavin by both free diffusion and direct transfer | Vibrio harveyi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| FMN + NADPH | reaction may be coupled with luciferase for bioluminescence | Vibrio harveyi | FMNH2 + NADP+ | - |
? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | - |
Vibrio harveyi | |
| NADPH | - |
Vibrio harveyi | |
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Release 2023.1 (January 2023)
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