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Literature summary for 1.5.1.3 extracted from
- Comprehensive characterization of dihydrofolate reductase-mediated gene amplification for the establishment of recombinant human embryonic kidney 293 cells producing monoclonal antibodies (2021), Biotechnol. J., 16, e2000351 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | application of HEK-293 cells as an alternative host cell line for stable expression of therapeutic glycoproteins. DHFR-deficient cells are generated by disrupting both DHFR and DHFR1 in HEK-293E cells and an expression vector containing DHFR and monoclonal antibody gene is transfected into the disruption mutant. A stable high-producing recombinant HEK293 cell line can be established using DHFR/methotrexate-mediated gene amplification | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P00374 | - |
- |
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Release 2023.1 (January 2023)
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