Cloned (Comment) | Organism |
---|---|
gene dhfr-ts, DNA and amino acid sequence determination and analysis, a 1,548-bp open reading frame encoding a 58.8-kDa translated peptide containing DHFR and TS domains linked together in a single polypeptide chain, expression of the GST-tagged enzyme in Escherichia coli strain BL21(DE3) as soluble protein, expression as GFP-tagged enzyme in Babesia gibsoni cytoplasm | Babesia gibsoni |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
methotrexate | - |
Babesia gibsoni | |
pyrimethamine | - |
Babesia gibsoni | |
trimethoprim | - |
Babesia gibsoni |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0016 | - |
7,8-dihydrofolate | 25°C, recombinant GST-tagged enzyme | Babesia gibsoni | |
0.0047 | - |
7,8-dihydrofolate | 25°C, recombinant detagged enzyme | Babesia gibsoni | |
0.00975 | - |
NADPH | 25°C, recombinant detagged enzyme | Babesia gibsoni | |
0.017 | - |
NADPH | 25°C, recombinant GST-tagged enzyme | Babesia gibsoni |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Babesia gibsoni | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
x * 57000, recombinant detagged enzyme, SDS-PAGE | Babesia gibsoni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH + H+ | Babesia gibsoni | - |
5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Babesia gibsoni | B5U9U8 | gene dhfr-ts | - |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged enzyme from Escherichia coli by glutathione affinity chromatography, the tag is cleaved off | Babesia gibsoni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH + H+ | - |
Babesia gibsoni | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
additional information | a bifunctional enzyme with dihydrofolate reductase and thymidylate synthase activities, overview | Babesia gibsoni | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 57000, recombinant detagged enzyme, SDS-PAGE | Babesia gibsoni |
Synonyms | Comment | Organism |
---|---|---|
DHFR-TS | - |
Babesia gibsoni |
dihydrofolate reductase-thymidylate synthase | - |
Babesia gibsoni |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Babesia gibsoni |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Babesia gibsoni |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | antifolate inhibition kinetics, overview | Babesia gibsoni |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0000686 | - |
25°C, recombinant detagged enzyme | Babesia gibsoni | methotrexate | |
0.05 | - |
25°C, recombinant detagged enzyme | Babesia gibsoni | trimethoprim | |
0.055 | - |
25°C, recombinant detagged enzyme | Babesia gibsoni | pyrimethamine |