Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Human gammaherpesvirus 8 |
Protein Variants | Comment | Organism |
---|---|---|
additional information | C-terminal-truncated mutant with lower activity | Human gammaherpesvirus 8 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
7,8-dihydrofolate | substrate inhibition at concentration above 0.008 mM for native enzyme and at concentration above 0.001 mM for the recombinant enzyme | Human gammaherpesvirus 8 | |
aminopterin | - |
Homo sapiens | |
aminopterin | - |
Human gammaherpesvirus 8 | |
methotrexate | recombinant enzyme | Homo sapiens | |
methotrexate | - |
Human gammaherpesvirus 8 | |
piritrexim | recombinant enzyme | Homo sapiens | |
piritrexim | - |
Human gammaherpesvirus 8 | |
pyrimethamine | recombinant enzyme | Homo sapiens | |
pyrimethamine | - |
Human gammaherpesvirus 8 | |
trimethoprim | recombinant enzyme | Homo sapiens | |
trimethoprim | - |
Human gammaherpesvirus 8 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00039 | - |
7,8-dihydrofolate | recombinant enzyme | Homo sapiens | |
0.001 | - |
NADPH | for native and recombinant enzymes, monophasic | Homo sapiens | |
0.001 | - |
NADPH | for native and recombinant enzymes, monophasic | Human gammaherpesvirus 8 | |
0.0024 | - |
7,8-dihydrofolate | native enzyme | Human gammaherpesvirus 8 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | wild-type and recombinant viral enzyme in transfected cells | Human gammaherpesvirus 8 | 5634 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
methotrexate-resistant WIL-2 lymphoblastoid cells | - |
Human gammaherpesvirus 8 | - |
human virus produced in cultured lymphoma derived B-cells | - |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli | Human gammaherpesvirus 8 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH | - |
Homo sapiens | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
7,8-dihydrofolate + NADPH | - |
Human gammaherpesvirus 8 | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7 | - |
7,8-dihydrofolate | native enzyme | Human gammaherpesvirus 8 | |
16.8 | - |
7,8-dihydrofolate | recombinant enzyme | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Homo sapiens | |
NADPH | - |
Human gammaherpesvirus 8 |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Homo sapiens | |
additional information | - |
additional information | Ki-values for the native enzyme are higher than for the recombinant enzyme | Human gammaherpesvirus 8 | |
0.000000009 | - |
methotrexate | recombinant enzyme | Homo sapiens | |
0.0000002 | - |
methotrexate | - |
Human gammaherpesvirus 8 | |
0.00000061 | - |
aminopterin | - |
Human gammaherpesvirus 8 | |
0.000001 | - |
pyrimethamine | recombinant enzyme | Homo sapiens | |
0.0000039 | - |
piritrexim | - |
Human gammaherpesvirus 8 | |
0.000008 | - |
piritrexim | recombinant enzyme | Homo sapiens | |
0.000029 | - |
pyrimethamine | - |
Human gammaherpesvirus 8 | |
0.00081 | - |
trimethoprim | recombinant enzyme | Homo sapiens | |
0.0023 | - |
trimethoprim | - |
Human gammaherpesvirus 8 |