General Stability | Organism |
---|---|
N5-formyltetrahydrofolate, NADP+ and NADPH stabilize and protect against heat inactivation | Gallus gallus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
aminopterin | - |
Gallus gallus | |
citrate | in acid pH range: inhibitor of reduction of 7,8-dihydrofolate but not folate | Gallus gallus | |
NADP+ | - |
Gallus gallus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00015 | - |
7,8-dihydrofolate | with NADPH | Gallus gallus | |
0.0067 | - |
folate | - |
Gallus gallus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
sedimentation equilibrium studies, sucrose density gradient experiments, aminopterin titration | Gallus gallus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Gallus gallus | - |
Storage Stability | Organism |
---|---|
-60°C, 0.02 mM NADPH, 3 months, frequent freezing and thawing, stable | Gallus gallus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH | - |
Gallus gallus | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
folate + NADPH | - |
Gallus gallus | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
half-life: 2 min | Gallus gallus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5 | - |
7,8-dihydrofolate | pH 7.4 | Gallus gallus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.8 | - |
folate + NADPH | Gallus gallus |
4 | - |
7,8-dihydrofolate + NADPH, 2 optima: pH 4 and pH 7.4 | Gallus gallus |
5.2 | 5.5 | 7,8-dihydrofolate + NADH | Gallus gallus |
7.4 | - |
7,8-dihydrofolate + NADPH, 2 optima: pH 4.0 and pH 7.4 | Gallus gallus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | maximal stability | Gallus gallus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | chicken liver: at neutral pH specific for NADPH and 7,8-dihydrofolate, at acid pH: NADH + 7,8-dihydrofolate and folate + NADPH | Gallus gallus | |
NADPH | - |
Gallus gallus |