Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Cl- | - |
Homo sapiens | |
Urea | - |
Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-(N-[(2,4-Diamino-6-pteridyl)methyl]-N-methylamino)benzoate | - |
Homo sapiens | |
aminopterin | - |
Homo sapiens | |
Dichloromethotrexate | - |
Homo sapiens | |
folate | - |
Homo sapiens | |
methotrexate | dihydrofolate antagonist drug | Homo sapiens | |
p-hydroxymercuribenzoate | - |
Homo sapiens | |
Pteroate | - |
Homo sapiens | |
pyrimethamine | - |
Homo sapiens | |
Triampterene | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | stimulation | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | 22000 | human placenta, gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH | Homo sapiens | key role in thymidylate biosynthesis | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
methotrexate-resistant WIL-2 lymphoblastoid cells | - |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
placenta | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
17.5 | - |
purified enzyme | Homo sapiens |
Storage Stability | Organism |
---|---|
-20°C, 50% glycerol, 0.1 M potassium phosphate, pH 7.0, less than 10% loss of activity, 6 months | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH | - |
Homo sapiens | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
7,8-dihydrofolate + NADPH | key role in thymidylate biosynthesis | Homo sapiens | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
folate + NADPH | - |
Homo sapiens | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.63 | - |
7,8-dihydrofolate | pH 6.0 | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.2 | - |
2 optima: pH 4.2 and pH 7.5 | Homo sapiens |
7.5 | - |
2 optima: pH 4.2 and pH 7.5 | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | low activity | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | overview | Homo sapiens | |
0.0000000061 | - |
methotrexate | - |
Homo sapiens | |
0.0000000078 | - |
aminopterin | - |
Homo sapiens | |
0.00000078 | - |
4-(N-[(2,4-Diamino-6-pteridyl)methyl]-N-methylamino)benzoate | - |
Homo sapiens | |
0.0000022 | - |
pyrimethamine | - |
Homo sapiens | |
0.00002 | - |
Triampterene | - |
Homo sapiens | |
0.000026 | - |
folate | - |
Homo sapiens | |
0.0018 | - |
Pteroate | - |
Homo sapiens |