Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.5.1.3 extracted from

  • Meek, T.D.; Garvey, E.P.; Santi, D.V.
    Purification and characterization of the bifunctional thymidylate synthetase-dihydrofolate reductase from methotrexate-resistant Leishmania tropica (1985), Biochemistry, 24, 678-686.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
5-methyltetrahydrofolate
-
Leishmania tropica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Leishmania tropica
0.0015
-
7,8-dihydrofolate
-
Leishmania tropica
0.0027
-
NADPH
-
Leishmania tropica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56400
-
2 * 56400, methotrexate-resistant, each subunit is a bifunctional thymidylate synthase-dihydrofolate reductase, SDS-PAGE Leishmania tropica
110000
-
methotrexate resistant, bifunctional, glycerol density gradient centrifugation Leishmania tropica

Organism

Organism UniProt Comment Textmining
Leishmania tropica
-
methotrexate resistant bifunctional enzyme: thymidylate synthase-dihydrofolate reductase
-

Purification (Commentary)

Purification (Comment) Organism
-
Leishmania tropica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Leishmania tropica

Storage Stability

Storage Stability Organism
-20°C, 40% glycerol, pH 7.4, 5 mM dithiothreitol, 1 mM EDTA, 1 month Leishmania tropica
4°C, 40-50% loss of activity after 1 month Leishmania tropica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7,8-dihydrofolate + NADPH
-
Leishmania tropica 5,6,7,8-tetrahydrofolate + NADP+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 56400, methotrexate-resistant, each subunit is a bifunctional thymidylate synthase-dihydrofolate reductase, SDS-PAGE Leishmania tropica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Leishmania tropica

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.15
-
5-methyltetrahydrofolate
-
Leishmania tropica