General Stability | Organism |
---|---|
freezing and thawing, significant loss of activity even in presence of glycerol | Glycine max |
glycerol, 25%, essential for stabilization during purification | Glycine max |
protease inhibitors phenylmethylsulfonyl fluoride and leupeptin are essential for stabilization during purification | Glycine max |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,4-diaminoquinazoline | derivatives | Glycine max | |
folate | - |
Glycine max | |
K+ | - |
Glycine max | |
methotrexate | - |
Glycine max | |
pyrimethamine | - |
Glycine max | |
trimethoprim | - |
Glycine max | |
Urea | - |
Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
7,8-dihydrofolate | - |
Glycine max | |
0.03 | - |
NADPH | - |
Glycine max |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
organic mercurials | no activation | Glycine max |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | - |
SDS-PAGE | Glycine max |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Glycine max | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15 | - |
crude extract | Glycine max |
56.5 | - |
purified enzyme | Glycine max |
Storage Stability | Organism |
---|---|
4°C, 0.1 M imidazole, pH 7.2, 25% glycerol, stable | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH | - |
Glycine max | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | ? * 22000, SDS-PAGE | Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Glycine max |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Glycine max |