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Literature summary for 1.5.1.3 extracted from

  • Ratnam, S.; Delcamp, T.J.; Hynes, J.B.; Freisheim, J.H.
    Purification and characterization of dihydrofolate reductase from soybean seedlings (1987), Arch. Biochem. Biophys., 255, 279-289.
    View publication on PubMed

General Stability

General Stability Organism
freezing and thawing, significant loss of activity even in presence of glycerol Glycine max
glycerol, 25%, essential for stabilization during purification Glycine max
protease inhibitors phenylmethylsulfonyl fluoride and leupeptin are essential for stabilization during purification Glycine max

Inhibitors

Inhibitors Comment Organism Structure
2,4-diaminoquinazoline derivatives Glycine max
folate
-
Glycine max
K+
-
Glycine max
methotrexate
-
Glycine max
pyrimethamine
-
Glycine max
trimethoprim
-
Glycine max
Urea
-
Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
7,8-dihydrofolate
-
Glycine max
0.03
-
NADPH
-
Glycine max

Metals/Ions

Metals/Ions Comment Organism Structure
organic mercurials no activation Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
SDS-PAGE Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15
-
crude extract Glycine max
56.5
-
purified enzyme Glycine max

Storage Stability

Storage Stability Organism
4°C, 0.1 M imidazole, pH 7.2, 25% glycerol, stable Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7,8-dihydrofolate + NADPH
-
Glycine max 5,6,7,8-tetrahydrofolate + NADP+
-
?

Subunits

Subunits Comment Organism
? ? * 22000, SDS-PAGE Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
Glycine max

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Glycine max