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Literature summary for 1.5.1.3 extracted from

  • Nixon, P.F.; Blakley, R.J.
    Dihydrofolate reductase of Streptococcus faecium. II. Purification and some properties of two dihydrofolate reductases from the amethopterin-resistant mutant Streptococcus faecium var. durans strain A (1968), J. Biol. Chem., 243, 4722-4731.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
NH4+ activation of mutant type enzyme at 0.2 mM, decrease of wild type enzyme activity above 0.1 mM Enterococcus faecium

Inhibitors

Inhibitors Comment Organism Structure
Cs+ activation of mutant type enzyme at 0.2 mM, decrease of wild-type enzyme activity above 0.1 mM Enterococcus faecium
K+ activation of mutant type enzyme at 0.2 mM, decrease of wild-type enzyme activity above 0.1 mM Enterococcus faecium
Li+ activation of mutant type enzyme at 0.2 mM, decrease of wild-type enzyme activity above 0.1 mM Enterococcus faecium
methopterin
-
Enterococcus faecium
Na+ activation of mutant type enzyme at 0.2 mM, decrease of wild-type enzyme activity above 0.1 mM Enterococcus faecium
NH4+ activation of mutant type enzyme at 0.2 mM, decrease of wild-type enzyme activity above 0.1 mM Enterococcus faecium
p-mercuribenzoate mutant enzyme: no effect, wild type: inhibition Enterococcus faecium
phenylmercuric acetate mutant enzyme: no effect, wild type enzyme: inhibition Enterococcus faecium
Rb+ activation of mutant type enzyme at 0.2 mM, decrease of wild-type enzyme activity above 0.1 mM Enterococcus faecium
Urea inactivation Enterococcus faecium

Metals/Ions

Metals/Ions Comment Organism Structure
Cs+ activation of mutant type enzyme at 0.2 mM, decrease of wild type enzyme activity above 0.1 mM Enterococcus faecium
K+ activation of mutant type enzyme activity at 0.2 mM, decrease of wild type enzyme activity above 0.1 mM Enterococcus faecium
Li+ activation of mutant type enzyme at 0.2 mM, decrease of wild type enzyme activity above 0.1 mM Enterococcus faecium
Na+ activation of mutant type enzyme 0.2 mM, decrease of wild type enzyme activity above 0.1 mM Enterococcus faecium
Rb+ activation of mutant type enzyme at 0.2 mM, decrease of wild type enzyme activity above 0.1 mM Enterococcus faecium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
velocity sedimentation, mutant type Enterococcus faecium
28000
-
wild type, gel filtration and velocity sedimentation Enterococcus faecium

Organism

Organism UniProt Comment Textmining
Enterococcus faecium
-
2 forms termed mutant type and wild-type enzyme in a single organism
-
Enterococcus faecium
-
var. durans
-
Enterococcus faecium
-
amethopterin-resistant strain
-
Enterococcus faecium A
-
var. durans
-

Purification (Commentary)

Purification (Comment) Organism
2 forms: mutant-type and wild type enzyme in a single organism Enterococcus faecium
amethopterin-resistant strain Enterococcus faecium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
285
-
-
Enterococcus faecium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7,8-dihydrofolate + NADPH
-
Enterococcus faecium 5,6,7,8-tetrahydrofolate + NADP+
-
?
7,8-dihydrofolate + NADPH
-
Enterococcus faecium A 5,6,7,8-tetrahydrofolate + NADP+
-
?
folate + NADPH
-
Enterococcus faecium 5,6,7,8-tetrahydrofolate + NADP+
-
?
folate + NADPH
-
Enterococcus faecium A 5,6,7,8-tetrahydrofolate + NADP+
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.125
-
folate mutant type Enterococcus faecium
15
-
7,8-dihydrofolate mutant type Enterococcus faecium
133
-
7,8-dihydrofolate wild-type Enterococcus faecium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.7
-
folate, mutant type enzyme Enterococcus faecium
5.8
-
wild type, mutant type Enterococcus faecium

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
-
Enterococcus faecium

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Enterococcus faecium