Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Arthrobacter sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) | 0.13 mM, complete inhibition | Arthrobacter sp. | |
Ag+ | 0.13 mM, complete inhibition | Arthrobacter sp. | |
Cd2+ | 0.13 mM, complete inhibition | Arthrobacter sp. | |
Co2+ | - |
Arthrobacter sp. | |
Cu2+ | 0.13 mM, complete inhibition | Arthrobacter sp. | |
Fe2+ | - |
Arthrobacter sp. | |
Hg2+ | 0.13 mM, complete inhibition | Arthrobacter sp. | |
Mn2+ | - |
Arthrobacter sp. | |
N-[1-S-(Carboxy)ethyl]-(S)-Phe | - |
Arthrobacter sp. | |
NEM | 0.13 mM, complete inhibition | Arthrobacter sp. | |
Ni2+ | 0.13 mM, complete inhibition | Arthrobacter sp. | |
Pb2+ | - |
Arthrobacter sp. | |
PCMB | - |
Arthrobacter sp. | |
Zn2+ | - |
Arthrobacter sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.5 | - |
L-Cys | pyruvate and NADH as cosubstrates | Arthrobacter sp. | |
2.9 | - |
L-Leu | - |
Arthrobacter sp. | |
3 | - |
pyruvate | L-Met and NADH as cosubstrate | Arthrobacter sp. | |
3.5 | - |
oxaloacetate | L-Met and NADH as cosubstrate | Arthrobacter sp. | |
3.6 | - |
L-Ser | pyruvate and NADH as cosubstrates | Arthrobacter sp. | |
4.1 | - |
L-Met | pyruvate and NADH as cosubstrates | Arthrobacter sp. | |
5.1 | - |
L-Ala | pyruvate and NADH as cosubstrates | Arthrobacter sp. | |
6.2 | - |
L-Ile | pyruvate and NADH as cosubstrates | Arthrobacter sp. | |
7.4 | - |
L-Thr | pyruvate and NADH as cosubstrates | Arthrobacter sp. | |
7.5 | - |
L-Phe | pyruvate and NADH as cosubstrates | Arthrobacter sp. | |
8.8 | - |
glyoxylate | L-Met and NADH as cosubstrate | Arthrobacter sp. | |
28 | - |
2-Oxohexanoate | L-Met and NADH as cosubstrate | Arthrobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
gel filtration | Arthrobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter sp. | - |
- |
- |
Arthrobacter sp. 1C | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Arthrobacter sp. |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Arthrobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gly + pyruvate + NADH | 5.3% of the activity with L-Met | Arthrobacter sp. | N-(carboxymethyl)-D-alanine + NAD+ | - |
r | |
L-Ala + pyruvate + NADH | 67% of the activity with L-Met | Arthrobacter sp. | N-[1-(R)-(Carboxy)ethyl]-(S)-Ala + NAD+ | - |
r | |
L-Asp + pyruvate + NADH | 1.2% of the activity with L-Met | Arthrobacter sp. | N-[1-(R)-(Carboxy)ethyl]-(S)-Asp + NAD+ | - |
r | |
L-Cys + pyruvate + NADH | 39% of the activity with L-Met | Arthrobacter sp. | N-[1-(R)-(Carboxy)ethyl]-(S)-Cys + NAD+ | - |
r | |
L-Ile + pyruvate + NADH | 94% of the activity with L-Met | Arthrobacter sp. | N-[1-(R)-(Carboxy)ethyl]-(S)-Ile + NAD+ | - |
r | |
L-Ile + pyruvate + NADH | 94% of the activity with L-Met | Arthrobacter sp. 1C | N-[1-(R)-(Carboxy)ethyl]-(S)-Ile + NAD+ | - |
r | |
L-Leu + pyruvate + NADH | 89% of the activity with L-Met | Arthrobacter sp. | N-[1-(R)-(Carboxy)ethyl]-(S)-Leu + NAD+ | - |
r | |
L-Leu + pyruvate + NADH | 89% of the activity with L-Met | Arthrobacter sp. 1C | N-[1-(R)-(Carboxy)ethyl]-(S)-Leu + NAD+ | - |
r | |
L-Met + 2-oxobutanoate + NADH | 11% of the activity with pyruvate | Arthrobacter sp. | ? | - |
r | |
L-Met + 2-oxohexanoate + NADH | 2.1% of the activity with pyruvate | Arthrobacter sp. | ? | - |
r | |
L-Met + glyoxylate + NADH | 11% of the activity with pyruvate | Arthrobacter sp. | ? | - |
r | |
L-Met + hydroxypyruvate + NADH | 6.1% of the activity with pyruvate | Arthrobacter sp. | ? | - |
r | |
L-Met + oxaloacetate + NADH | 30% of the activity with pyruvate | Arthrobacter sp. | ? | - |
r | |
L-Met + pyruvate + NADH | - |
Arthrobacter sp. | N-[1-(R)-(Carboxy)ethyl]-(S)-Met + NAD+ | - |
r | |
L-Met + pyruvate + NADH | - |
Arthrobacter sp. 1C | N-[1-(R)-(Carboxy)ethyl]-(S)-Met + NAD+ | - |
r | |
L-Phe + pyruvate + NADH | 91% of the activity with L-Met | Arthrobacter sp. | N-[1-(R)-(Carboxy)ethyl]-(S)-Phe + NAD+ | - |
? | |
L-Phe + pyruvate + NADH | 91% of the activity with L-Met | Arthrobacter sp. 1C | N-[1-(R)-(Carboxy)ethyl]-(S)-Phe + NAD+ | - |
? | |
L-Ser + pyruvate + NADH | 39% of the activity with L-Met | Arthrobacter sp. | N-[1-(R)-(Carboxy)ethyl]-(S)-Ser + NAD+ | - |
r | |
L-Thr + pyruvate + NADH | 50% of the activity with L-Met | Arthrobacter sp. | N-[1-(R)-(Carboxy)ethyl]-(S)-Thr + NAD+ | - |
r | |
L-Val + pyruvate + NADH | 92% of the activity with L-Met | Arthrobacter sp. | N-[1-(R)-(Carboxy)ethyl]-(S)-Val + NAD+ | - |
r | |
L-Val + pyruvate + NADH | 92% of the activity with L-Met | Arthrobacter sp. 1C | N-[1-(R)-(Carboxy)ethyl]-(S)-Val + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Arthrobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Arthrobacter sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
pH 5.0-9.5, 1 h, less than 15% loss of activity | Arthrobacter sp. |
37 | - |
pH 8.0, 10 min, stable | Arthrobacter sp. |
50 | - |
pH 8.0, 10 min, 20% loss of activity | Arthrobacter sp. |
52 | - |
pH 8.0, 10 min, 50% loss of activity | Arthrobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
amine-forming reaction | Arthrobacter sp. |
10 | - |
oxidative deamination | Arthrobacter sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9.5 | 30°C, 1 h less than 15% loss of activity | Arthrobacter sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Arthrobacter sp. |