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Literature summary for 1.5.1.28 extracted from

  • Asano, Y.; Yamaguchi, K.; Kondo, K.
    A new NAD+-dependent opine dehydrogenase from Arthrobacter sp. strain 1C (1989), J. Bacteriol., 171, 4466-4471.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
NADH
-
Arthrobacter sp.

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoic acid) 0.13 mM, complete inhibition Arthrobacter sp.
Ag+ 0.13 mM, complete inhibition Arthrobacter sp.
Cd2+ 0.13 mM, complete inhibition Arthrobacter sp.
Co2+
-
Arthrobacter sp.
Cu2+ 0.13 mM, complete inhibition Arthrobacter sp.
Fe2+
-
Arthrobacter sp.
Hg2+ 0.13 mM, complete inhibition Arthrobacter sp.
Mn2+
-
Arthrobacter sp.
N-[1-S-(Carboxy)ethyl]-(S)-Phe
-
Arthrobacter sp.
NEM 0.13 mM, complete inhibition Arthrobacter sp.
Ni2+ 0.13 mM, complete inhibition Arthrobacter sp.
Pb2+
-
Arthrobacter sp.
PCMB
-
Arthrobacter sp.
Zn2+
-
Arthrobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5
-
L-Cys pyruvate and NADH as cosubstrates Arthrobacter sp.
2.9
-
L-Leu
-
Arthrobacter sp.
3
-
pyruvate L-Met and NADH as cosubstrate Arthrobacter sp.
3.5
-
oxaloacetate L-Met and NADH as cosubstrate Arthrobacter sp.
3.6
-
L-Ser pyruvate and NADH as cosubstrates Arthrobacter sp.
4.1
-
L-Met pyruvate and NADH as cosubstrates Arthrobacter sp.
5.1
-
L-Ala pyruvate and NADH as cosubstrates Arthrobacter sp.
6.2
-
L-Ile pyruvate and NADH as cosubstrates Arthrobacter sp.
7.4
-
L-Thr pyruvate and NADH as cosubstrates Arthrobacter sp.
7.5
-
L-Phe pyruvate and NADH as cosubstrates Arthrobacter sp.
8.8
-
glyoxylate L-Met and NADH as cosubstrate Arthrobacter sp.
28
-
2-Oxohexanoate L-Met and NADH as cosubstrate Arthrobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
gel filtration Arthrobacter sp.

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
-
-
Arthrobacter sp. 1C
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Arthrobacter sp.

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gly + pyruvate + NADH 5.3% of the activity with L-Met Arthrobacter sp. N-(carboxymethyl)-D-alanine + NAD+
-
r
L-Ala + pyruvate + NADH 67% of the activity with L-Met Arthrobacter sp. N-[1-(R)-(Carboxy)ethyl]-(S)-Ala + NAD+
-
r
L-Asp + pyruvate + NADH 1.2% of the activity with L-Met Arthrobacter sp. N-[1-(R)-(Carboxy)ethyl]-(S)-Asp + NAD+
-
r
L-Cys + pyruvate + NADH 39% of the activity with L-Met Arthrobacter sp. N-[1-(R)-(Carboxy)ethyl]-(S)-Cys + NAD+
-
r
L-Ile + pyruvate + NADH 94% of the activity with L-Met Arthrobacter sp. N-[1-(R)-(Carboxy)ethyl]-(S)-Ile + NAD+
-
r
L-Ile + pyruvate + NADH 94% of the activity with L-Met Arthrobacter sp. 1C N-[1-(R)-(Carboxy)ethyl]-(S)-Ile + NAD+
-
r
L-Leu + pyruvate + NADH 89% of the activity with L-Met Arthrobacter sp. N-[1-(R)-(Carboxy)ethyl]-(S)-Leu + NAD+
-
r
L-Leu + pyruvate + NADH 89% of the activity with L-Met Arthrobacter sp. 1C N-[1-(R)-(Carboxy)ethyl]-(S)-Leu + NAD+
-
r
L-Met + 2-oxobutanoate + NADH 11% of the activity with pyruvate Arthrobacter sp. ?
-
r
L-Met + 2-oxohexanoate + NADH 2.1% of the activity with pyruvate Arthrobacter sp. ?
-
r
L-Met + glyoxylate + NADH 11% of the activity with pyruvate Arthrobacter sp. ?
-
r
L-Met + hydroxypyruvate + NADH 6.1% of the activity with pyruvate Arthrobacter sp. ?
-
r
L-Met + oxaloacetate + NADH 30% of the activity with pyruvate Arthrobacter sp. ?
-
r
L-Met + pyruvate + NADH
-
Arthrobacter sp. N-[1-(R)-(Carboxy)ethyl]-(S)-Met + NAD+
-
r
L-Met + pyruvate + NADH
-
Arthrobacter sp. 1C N-[1-(R)-(Carboxy)ethyl]-(S)-Met + NAD+
-
r
L-Phe + pyruvate + NADH 91% of the activity with L-Met Arthrobacter sp. N-[1-(R)-(Carboxy)ethyl]-(S)-Phe + NAD+
-
?
L-Phe + pyruvate + NADH 91% of the activity with L-Met Arthrobacter sp. 1C N-[1-(R)-(Carboxy)ethyl]-(S)-Phe + NAD+
-
?
L-Ser + pyruvate + NADH 39% of the activity with L-Met Arthrobacter sp. N-[1-(R)-(Carboxy)ethyl]-(S)-Ser + NAD+
-
r
L-Thr + pyruvate + NADH 50% of the activity with L-Met Arthrobacter sp. N-[1-(R)-(Carboxy)ethyl]-(S)-Thr + NAD+
-
r
L-Val + pyruvate + NADH 92% of the activity with L-Met Arthrobacter sp. N-[1-(R)-(Carboxy)ethyl]-(S)-Val + NAD+
-
r
L-Val + pyruvate + NADH 92% of the activity with L-Met Arthrobacter sp. 1C N-[1-(R)-(Carboxy)ethyl]-(S)-Val + NAD+
-
r

Subunits

Subunits Comment Organism
dimer
-
Arthrobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Arthrobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
pH 5.0-9.5, 1 h, less than 15% loss of activity Arthrobacter sp.
37
-
pH 8.0, 10 min, stable Arthrobacter sp.
50
-
pH 8.0, 10 min, 20% loss of activity Arthrobacter sp.
52
-
pH 8.0, 10 min, 50% loss of activity Arthrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
amine-forming reaction Arthrobacter sp.
10
-
oxidative deamination Arthrobacter sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9.5 30°C, 1 h less than 15% loss of activity Arthrobacter sp.

Cofactor

Cofactor Comment Organism Structure
NADH
-
Arthrobacter sp.