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Literature summary for 1.5.1.25 extracted from

  • Nardini, M.; Ricci, G.; Caccuri, A.M.; Solinas, S.P.; Vesci, L.; Cavallini, D.
    Purification and characterization of a ketimine-reducing enzyme (1988), Eur. J. Biochem., 173, 689-694.
    View publication on PubMed

General Stability

General Stability Organism
irreversible inactivation at protein concentration below 1 mg/ml. Complete loss of activity after 24 h at 4°C. Higher protein concentrations or 10% glycerol prevent enzyme inactivation. 30% loss of activity aftter 7 days at 4°C Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
Triton X-100 irreversible inactivation Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.027
-
NADH reaction with S-aminoethylcysteine ketimine Sus scrofa
0.077
-
S-Aminoethylcysteine ketimine
-
Sus scrofa
0.47
-
Lanthionine ketimine reaction with NADH Sus scrofa
3
-
Cystathionine ketimine reaction with NADH Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
73000
-
gel filtration Sus scrofa
76000
-
non-denaturing PAGE Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sus scrofa

Reaction

Reaction Comment Organism Reaction ID
thiomorpholine 3-carboxylate + NAD(P)+ = 3,4-dehydro-thiomorpholine-3-carboxylate + NAD(P)H + H+ classical ping-pong mechanism Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16.3
-
-
Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cystathionine ketimine + NADH
-
Sus scrofa cyclothionine + NAD+
-
ir
cystathionine ketimine + NADPH
-
Sus scrofa cyclothionine + NADP+
-
ir
DELTA1-piperideine 2-carboxylate + NADH
-
Sus scrofa ? + NAD+
-
ir
DELTA1-piperideine 2-carboxylate + NADPH
-
Sus scrofa ? + NADP+
-
ir
lanthionine ketimine + NADH
-
Sus scrofa 1,4-thiomorpholine 3,5-dicarboxylic acid + NAD+
-
ir
lanthionine ketimine + NADPH
-
Sus scrofa 1,4-thiomorpholine 3,5-dicarboxylic acid + NADP+
-
ir
S-aminoethylcysteine ketimine + NADH
-
Sus scrofa 1,4-thiomorpholine 3-carboxylic acid + NAD+ L-enantiomer ir
S-aminoethylcysteine ketimine + NADPH
-
Sus scrofa 1,4-thiomorpholine 3-carboxylic acid + NADP+
-
ir

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
reaction with S-aminoethylcysteine ketimine or lanthionine ketimine, acetate buffer or phosphate buffer Sus scrofa
5
-
-
Sus scrofa
6
-
reaction with cystathionine ketimine, acetate buffer or phosphate buffer Sus scrofa

Cofactor

Cofactor Comment Organism Structure
NADH NADH and NADPH show equal activity with cystathionine ketimine as substrate. Reduction of lanthionine ketimine with NADPH is faster than reduction with NADH. Reduction of S-aminoethylcysteine with NADH is faster than reduction with NADPH Sus scrofa
NADPH NADH and NADPH show equal activity with cystathionine ketimine as substrate. Reduction of lanthionine ketimine with NADPH is faster than reduction with NADH. Reduction of S-aminoethylcysteine with NADH is faster than reduction with NADPH Sus scrofa