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Literature summary for 1.5.1.24 extracted from

  • Sackett, D.L.; Ruvinov, S.B.; Thompson, J.
    N5-(L-1-carboxyethyl)-L-ornithine synthase: Physical and spectral characterization of teh enzyme and its unusual low pKa fluorescent tyrosine residues (1999), Protein Sci., 8, 2121-2129.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
nutrition industrially important organism used widely as a starter in the dairy industry Lactococcus lactis

Cloned(Commentary)

Cloned (Comment) Organism
ceo gene encoding N5-(carboxyethyl)ornithine synthase isolated from transposon Tn5306 of Lactococcus lactis K1 cloned and sequenced, expression in Escherichia coli CEO571 Lactococcus lactis
gene ceo, encoding the tetrameric enzyme, cloned and sequenced, plasmid p493 containing ceo transformed into Escherichia coli TG1 Lactococcus lactis

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Lactococcus lactis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35320
-
deduced from amino acid sequence Lactococcus lactis
37000
-
4 * 37000, SDS-PAGE Lactococcus lactis
137000
-
XLA Lactococcus lactis
140000
-
native PAGE Lactococcus lactis

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme purified from the overproducing strain Escherichia coli CEO571 Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-ornithine + pyruvate + NADPH
-
Lactococcus lactis N5-(L-1-carboxyethyl)-L-ornithine + NADP+ + H2O
-
ir

Subunits

Subunits Comment Organism
tetramer 4 * 37000, SDS-PAGE Lactococcus lactis
tetramer 4* 35355, MALDI-MS Lactococcus lactis

Synonyms

Synonyms Comment Organism
CEOS
-
Lactococcus lactis