Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxobutanoate | slight substrate inhibition | Arabella iricolor | |
oxaloacetate | slight substrate inhibition | Arabella iricolor | |
pyruvate | substrate inhibition | Arabella iricolor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.036 | - |
NADH | - |
Arabella iricolor | |
0.051 | - |
NAD+ | - |
Arabella iricolor | |
0.34 | - |
pyruvate | - |
Arabella iricolor | |
3.3 | - |
2-oxobutanoate | - |
Arabella iricolor | |
4.8 | - |
tauropine | - |
Arabella iricolor | |
4.9 | - |
oxaloacetate | - |
Arabella iricolor | |
35.7 | - |
taurine | - |
Arabella iricolor |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43500 | - |
1 * 43500, SDS-PAGE | Arabella iricolor |
43700 | - |
gel filtration | Arabella iricolor |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Arabella iricolor | the enzyme is a dominant pyruvate reductase in this organism | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabella iricolor | - |
- |
- |
Purification (Comment) | Organism |
---|---|
2 enzyme forms: TaDH-1 and TaDH-2 | Arabella iricolor |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
878.3 | - |
- |
Arabella iricolor |
Storage Stability | Organism |
---|---|
4°C, 2 mM KH2PO4-NaOH, pH 7.2, 1 mM EDTA, 10 mM 2-mercaptoethanol, stable for at least 3 weeks | Arabella iricolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Aminomethane sulfonic acid + pyruvate + NADH | at 1.8% of the activity with taurine | Arabella iricolor | N2-(1-Carboxyethyl)aminomethanesulfonic acid + NAD+ + H2O | - |
? | |
Homotaurine + pyruvate + NADH | at 2.4% of the activity with taurine | Arabella iricolor | ? | - |
? | |
Hypotaurine + pyruvate + NADH | at 40% of the activity with taurine | Arabella iricolor | N2-(1-Carboxyethyl)aminoethanesulfinic acid + NAD+ + H2O | - |
? | |
additional information | the enzyme is a dominant pyruvate reductase in this organism | Arabella iricolor | ? | - |
? | |
Taurine + 2-oxobutanoate + NADH | - |
Arabella iricolor | ? | - |
? | |
Taurine + 2-oxohexanoate + NADH | at 4.8% of the activity with pyruvate | Arabella iricolor | ? | - |
? | |
Taurine + 2-oxopentanoate + NADH | at 7.8% of the activity with pyruvate | Arabella iricolor | ? | - |
? | |
Taurine + 3-hydroxypyruvate + NADH | at 21.2% of the activity with pyruvate | Arabella iricolor | ? | - |
? | |
Taurine + glyoxylate + NADH | at 10.9% of the activity with pyruvate | Arabella iricolor | ? | - |
? | |
taurine + oxaloacetate + NADH | - |
Arabella iricolor | ? | - |
? | |
Taurine + pyruvate + NADH | - |
Arabella iricolor | Tauropine + NAD+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 43500, SDS-PAGE | Arabella iricolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | 7.3 | tauropine formation, enzyme form TaDH-1 | Arabella iricolor |
8.5 | - |
tauropine oxidation | Arabella iricolor |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.9 | 50% of maximal activity at pH 5.5 and at pH 7.9, tauropine formation, enzyme form TaDH-1 | Arabella iricolor |
6.8 | 8.5 | 50% of maximal activity at pH 6.8, optimum at pH 8.5, tauropine oxidation, enzyme form TaDH-1 | Arabella iricolor |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Arabella iricolor |