Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetate | - |
Meretrix lusoria | |
D-lactate | - |
Meretrix lusoria | |
iminodiacetate | - |
Meretrix lusoria | |
L-lactate | - |
Meretrix lusoria | |
oxaloacetate | - |
Meretrix lusoria | |
succinate | - |
Meretrix lusoria |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.39 | - |
pyruvate | pH 7.5, 25°C | Meretrix lusoria | |
34.2 | - |
glycine | pH 7.5, 25°C | Meretrix lusoria | |
39.2 | - |
L-Ala | pH 7.5, 25°C | Meretrix lusoria | |
45.9 | - |
L-2-aminobutyrate | pH 7.5, 25°C | Meretrix lusoria | |
163 | - |
L-Ser | pH 7.5, 25°C | Meretrix lusoria | |
196 | - |
beta-Ala | pH 7.5, 25°C | Meretrix lusoria | |
303 | - |
L-Thr | pH 7.5, 25°C | Meretrix lusoria |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10% of enzymatic activity is inhibited at 10 mM | Meretrix lusoria | |
Cu2+ | 50% of enzymatic activity is inhibited at 1 mM | Meretrix lusoria | |
Fe3+ | inhibits enzyme at 0.2 mM | Meretrix lusoria | |
Mg2+ | 20% of enzymatic activity is inhibited at 10 mM | Meretrix lusoria | |
Mn2+ | 13% of enzymatic activity is inhibited at 1 mM | Meretrix lusoria | |
Zn2+ | inhibits enzyme at 0.6 mM | Meretrix lusoria |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
SDS-PAGE | Meretrix lusoria |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Meretrix lusoria | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purified over 470fold using ammonium sulfate fractionation, sephacryl S-100 chromatography, hydroxyapatite adsorption, DEAE-sepharose chromatography, Blue-sepharose chromatography | Meretrix lusoria |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Meretrix lusoria | - |
Storage Stability | Organism |
---|---|
4°C, glycerol, 2 weeks, 100% activity | Meretrix lusoria |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-Ala + pyruvate + NADH | - |
Meretrix lusoria | ? | - |
? | |
glycine + pyruvate + NADH | Gly and Ala are preferred substrates | Meretrix lusoria | D-strombine + NAD+ + H2O | - |
? | |
L-2-Aminobutyrate + pyruvate + NADH | - |
Meretrix lusoria | ? | - |
? | |
L-Ala + pyruvate + NADH | Gly and Ala are preferred substrates | Meretrix lusoria | ? | - |
? | |
L-Ser + pyruvate + NADH | - |
Meretrix lusoria | ? | - |
? | |
L-Thr + pyruvate + NADH | - |
Meretrix lusoria | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | gel filtration, SDS-PAGE analysis, 1 * 46000 | Meretrix lusoria |
Synonyms | Comment | Organism |
---|---|---|
SDH | - |
Meretrix lusoria |
strombine dehydrogenase | - |
Meretrix lusoria |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Meretrix lusoria |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 46 | temperature optimum | Meretrix lusoria |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
56 | - |
enzyme is heat labile, pretreatment at 56°C for 20 sec only retains 20% of its original activity | Meretrix lusoria |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Meretrix lusoria |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.4 | 7.6 | pH optimum | Meretrix lusoria |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.63 | - |
iminodiacetate | pH 7.5, 25°C | Meretrix lusoria | |
1.35 | - |
iminodiacetate | pH 6.5, 25°C | Meretrix lusoria | |
3.69 | - |
acetate | pH 7.5, 25°C | Meretrix lusoria | |
3.98 | - |
succinate | pH 6.5, 25°C | Meretrix lusoria | |
5.57 | - |
acetate | pH 6.5, 25°C | Meretrix lusoria | |
6.93 | - |
oxaloacetate | pH 7.5, 25°C | Meretrix lusoria | |
14.3 | - |
oxaloacetate | pH 6.5, 25°C | Meretrix lusoria | |
15.4 | - |
succinate | pH 7.5, 25°C | Meretrix lusoria | |
16.4 | - |
D-lactate | pH 6.5, 25°C | Meretrix lusoria | |
17 | - |
D-lactate | pH 7.5, 25°C | Meretrix lusoria | |
21.2 | - |
L-lactate | pH 6.5, 25°C | Meretrix lusoria | |
22.9 | - |
L-lactate | pH 7.5, 25°C | Meretrix lusoria |