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Literature summary for 1.5.1.22 extracted from

  • Endo, N.; Kan-No, N.; Nagahisa, E.
    Purification, characterization, and cDNA cloning of opine dehydrogenases from the polychaete rockworm Marphysa sanguinea (2007), Comp. Biochem. Physiol. B, 147B, 293-307.
No PubMed abstract available
Search for more literature for 1.5.1.22

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Marphysa sanguinea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 NADH apparent value, isozyme St/AlDH1, with 5 mM pyruvate and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C Marphysa sanguinea
0.07
-
 NADH apparent value, isozyme St/AlDH1, with 5 mM pyruvate and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C Marphysa sanguinea
0.85
-
 NAD+ apparent value, isozyme St/AlDH1, with 20 mM D-strombine, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C Marphysa sanguinea
0.87
-
 NAD+ apparent value, isozyme St/AlDH1, with 20 mM meso-alanopine, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C Marphysa sanguinea
0.96
-
 pyruvate apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C Marphysa sanguinea
0.97
-
 pyruvate apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C Marphysa sanguinea
9.07
-
 meso-Alanopine apparent value, isozyme St/AlDH1, with 1 mM NAD+, in 0.1 M PIPES-NaOH (pH 9.1), at 30°C Marphysa sanguinea
9.17
-
 D-strombine apparent value, isozyme St/AlDH1, with 1 mM NAD+, in 0.1 M PIPES-NaOH (pH 9.1), at 30°C Marphysa sanguinea
10.2
-
 oxaloacetate apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C Marphysa sanguinea
10.2
-
 oxaloacetate apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C Marphysa sanguinea
11.71
-
 2-Ketobutyrate apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C Marphysa sanguinea
12.77
-
 2-Ketobutyrate apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C Marphysa sanguinea
39.8
-
 L-Ala apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C Marphysa sanguinea
40.1
-
 Gly apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C Marphysa sanguinea
382.1
-
 L-Ser apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C Marphysa sanguinea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40600
-
 reduced and denatured enzyme, estimated by SDS-PAGE Marphysa sanguinea
42000
-
 native enzyme, estimated by SDS-PAGE Marphysa sanguinea
43350
-
 deduced from amino acid sequence from strombine/alanopine dehydrogenase Marphysa sanguinea

Organism

Organism UniProt Comment Textmining
Marphysa sanguinea A0A9I9
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-cellulose SH column chromatography, hydroxyapatite column chromatography, and Toyopearl Super Q-650S column chromatography Marphysa sanguinea

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
 Marphysa sanguinea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6
-
 crude extract, at 30°C Marphysa sanguinea
87
-
 after 29fold purification, at 30°C Marphysa sanguinea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gly + 2-ketobutyrate + NADH + H+ 8.9% activity compared to Gly + pyruvate + NADH with isozyme St/AlDH1 Marphysa sanguinea 2-[(carboxymethyl)amino]butanoic acid + NAD+ + H2O
-
 ?
Gly + glyoxylate + NADH + H+ 3.9% activity compared to Gly + pyruvate + NADH with isozyme St/AlDH1 Marphysa sanguinea [(carboxymethyl)amino]propanedioic acid + NAD+ + H2O
-
 ?
Gly + oxaloacetate + NADH + H+ 39.1% activity compared to Gly + pyruvate + NADH with isozyme St/AlDH1 Marphysa sanguinea N-carboxymethylglycine + NAD+ + H2O
-
 ?
Gly + pyruvate + NADH + H+ 94.6% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1 Marphysa sanguinea D-strombine + NAD+ + H2O
-
 ?
Gly + pyruvate + NADPH less than 0.5% activity compared to Gly + pyruvate + NADH with isozyme St/AlDH1 Marphysa sanguinea D-strombine + NADP+ + H2O
-
 ?
L-Ala + 2-ketobutyrate + NADH + H+ 8.9% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1 Marphysa sanguinea 2-[[(1S)-1-carboxyethyl]amino]butanoic acid + NAD+ + H2O
-
 ?
L-Ala + glyoxylate + NADH + H+ 4.0% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1 Marphysa sanguinea N-(carboxymethyl)-L-Ala + NAD+ + H2O
-
 ?
L-Ala + oxaloacetate + NADH + H+ 40.0% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1 Marphysa sanguinea [[(1S)-1-carboxyethyl]amino]propanedioic acid + NAD+ + H2O
-
 ?
L-Ala + pyruvate + NADH + H+ 100% activity Marphysa sanguinea meso-alanopine + NAD+ + H2O
-
 ?
L-Ala + pyruvate + NADPH less than 0.5% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1 Marphysa sanguinea meso-alanopine + NADP+ + H2O
-
 ?
L-Cys + pyruvate + NADH + H+ 1.7% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1 Marphysa sanguinea N-(1-carboxyethyl)-L-Cys + NAD+ + H2O
-
 ?
L-Ser + pyruvate + NADH + H+ 14.2% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1 Marphysa sanguinea N-(1-carboxyethyl)-L-Ser + NAD+ + H2O
-
 ?
L-Val + pyruvate + NADH + H+ 0.9% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1 Marphysa sanguinea N-(1-carboxyethyl)-L-Val + NAD+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
St/AlDH1 isozyme Marphysa sanguinea
St/AlDH2 isozyme Marphysa sanguinea
St/AlDH3 isozyme Marphysa sanguinea
strombine/alanopine dehydrogenase St/AlDH functions physiologically as strombine dehydrogenase Marphysa sanguinea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.86
-
 NAD+ apparent value, isozyme St/AlDH1, with 20 mM D-strombine, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C Marphysa sanguinea
3.96
-
 NAD+ apparent value, isozyme St/AlDH1, with 20 mM meso-alanopine, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C Marphysa sanguinea
4.99
-
 D-strombine apparent value, isozyme St/AlDH1, with 1 mM NAD+, in 0.1 M PIPES-NaOH (pH 9.1), at 30°C Marphysa sanguinea
5.12
-
 meso-Alanopine apparent value, isozyme St/AlDH1, with 1 mM NAD+, in 0.1 M PIPES-NaOH (pH 9.1), at 30°C Marphysa sanguinea
21.77
-
 2-Ketobutyrate apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C Marphysa sanguinea
23.2
-
 2-Ketobutyrate apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C Marphysa sanguinea
23.64
-
 L-Ser apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C Marphysa sanguinea
42.66
-
 oxaloacetate apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C Marphysa sanguinea
43.58
-
 NADH apparent value, isozyme St/AlDH1, with 5 mM pyruvate and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C Marphysa sanguinea
44.25
-
 oxaloacetate apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C Marphysa sanguinea
44.7
-
 NADH apparent value, isozyme St/AlDH1, with 5 mM pyruvate and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C Marphysa sanguinea
47.33
-
 pyruvate apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C Marphysa sanguinea
47.78
-
 pyruvate apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C Marphysa sanguinea
50.08
-
 Gly apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C Marphysa sanguinea
51.7
-
 L-Ala apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C Marphysa sanguinea

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Marphysa sanguinea