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Literature summary for 1.5.1.20 extracted from

  • Vickers, T.J.; Orsomando, G.; de la Garza, R.D.; Scott, D.A.; Kang, S.O.; Hanson, A.D.; Beverley, S.M.
    Biochemical and genetic analysis of methylenetetrahydrofolate reductase in Leishmania metabolism and virulence (2006), J. Biol. Chem., 281, 38150-38158.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Leishmania major

Protein Variants

Protein Variants Comment Organism
additional information enzyme null mutants lack 5-methyltetrahydrofolate and cannot utilize exogenous homocysteine for growth. In mouse model of infection, null mutants show good infectivity and virulence Leishmania major

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibitory: S-adenosyl-L-methionine, no substrate inhibition up to 0.25 mM Leishmania major

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34800
-
x * 34800, calculated Leishmania major

Organism

Organism UniProt Comment Textmining
Leishmania major
-
expression in Saccharomyces cerevisiae
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information protein sequence shows no obvious intracellular targeting sequences Leishmania major

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-methyltetrahydrofolate + NAD+
-
Leishmania major 5,10-methylenetetrahydrofolate + NADH
-
?
5-methyltetrahydrofolate + NADP+
-
Leishmania major 5,10-methylenetetrahydrofolate + NADPH
-
?
5-methyltetrahydrofolate + oxidized menadione
-
Leishmania major 5,10-methylenetetrahydrofolate + reduced menadione
-
?

Subunits

Subunits Comment Organism
? x * 34800, calculated Leishmania major

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
52
-
NADH phosphate buffer Leishmania major
74
-
NADPH phosphate buffer Leishmania major
87
-
NADH Tris buffer Leishmania major
140
-
NADPH Tris buffer Leishmania major

Cofactor

Cofactor Comment Organism Structure
NADH dual-cofactor specificity with NADH and NADPH under physiological conditions Leishmania major
NADPH dual-cofactor specificity with NADH and NADPH under physiological conditions Leishmania major