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Literature summary for 1.5.1.20 extracted from

  • Yamada, K.; Chen, Z.; Rozen, R.; Matthews, R.G.
    Effects of common polymorphisms on the properties of recombinant human methylenetetrahydrofolate reductase (2001), Proc. Natl. Acad. Sci. USA, 98, 14853-14858.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
medicine C677T mution of MTHRF gene is the most frequent genetic cause of mild hyperhomocysteinemia, a risk factor for cardiovascular disease Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of recombinant human MTHFR at high levels in Sf9 cells from Spodoptera frugiperda by using a baculovirus expression system Homo sapiens

Protein Variants

Protein Variants Comment Organism
A177V enzyme with decreased affinity for its FAD cofactor Escherichia coli
A222V most frequent genetic cause of mild hyperhomocysteinemia, enzyme with enhanced propensity to dissociate into monomers and to lose its FAD cofactor on dilution, increased thermolability of enzyme activity Homo sapiens
E429A A1298C mutation of the MTHRF gene, enzyme with indistinguishable properties from the wild-type Homo sapiens
additional information C677T mutation in MTHFR gene is a polymorphism which leads to the substitution of Ala-222 by valine Homo sapiens
additional information A1298C mutation of the MTHFR gene, which leads to the substitution of Glu-429 by alanine Homo sapiens

General Stability

General Stability Organism
methyltetrahydrofolate and S-adenosylmethionine protects enzyme from the loss of FAD after dilution Escherichia coli
methyltetrahydrofolate and S-adenosylmethionine protects enzyme from the loss of FAD after dilution Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
S-adenosylmethionine strong, reversible allosteric inhibition, prevented by S-adenosylhomocysteine Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
4 * 33000 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydrofolate + NADPH Homo sapiens physiological NADPH-CH2-H4folate oxidoreductase activity 5-methyltetrahydrofolate + NADP+ 5-methyltetrahydrofolate is the major methyl donor for the conversion of homocysteine to methionine ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
62fold purification of recombinant enzyme, expressed in Sf9 cells from Spodoptera frugiperda Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.4
-
recombinant enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + NADPH physiological NADPH-CH2-H4folate oxidoreductase activity Homo sapiens 5-methyltetrahydrofolate + NADP+ 5-methyltetrahydrofolate is the major methyl donor for the conversion of homocysteine to methionine ?
5,10-methylenetetrahydrofolate + reduced acceptor forward reaction: NADPH as reduced acceptor Escherichia coli 5-methyltetrahydrofolate + oxidized acceptor
-
?
5,10-methylenetetrahydrofolate + reduced acceptor forward reaction: NADPH as reduced acceptor Homo sapiens 5-methyltetrahydrofolate + oxidized acceptor
-
?
5,10-methylenetetrahydrofolate + reduced acceptor forward reaction: NADH as reduced acceptor Escherichia coli 5-methyltetrahydrofolate + oxidized acceptor
-
?

Subunits

Subunits Comment Organism
dimer
-
Homo sapiens
homotetramer 4 * 33000 Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
thermolability is enhanced when the FAD cofactor dissociates form enzyme Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein with FAD as cofactor Escherichia coli
FAD flavoprotein with FAD as cofactor Homo sapiens
FAD FAD is essential for electron transfer between NADH and methylenetetrahydrofolate Escherichia coli
NADH
-
Escherichia coli
NADPH
-
Escherichia coli
NADPH
-
Homo sapiens