Activating Compound | Comment | Organism | Structure |
---|---|---|---|
S-adenosylmethionine | stimulates | Arabidopsis sp. |
Cloned (Comment) | Organism |
---|---|
two MTHFR genes, a cDNAs encoding functional MTHFR is cloned, sequenced and expressed in Saccharomyces cerevisiae | Zea mays |
two MTHFR genes, two cDNAs encoding functional MTHFRs are cloned, sequenced and expressed in Saccharomyces cerevisiae at high levels | Arabidopsis sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by S-adenosylmethionine | Arabidopsis sp. | |
additional information | not inhibited by S-adenosylmethionine | Escherichia coli | |
additional information | not inhibited by S-adenosylmethionine | Zea mays |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Arabidopsis sp. | 5829 | - |
cytosol | - |
Zea mays | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
4 * 33000 | Escherichia coli |
66000 | - |
2 * 66000, SDS-PAGE | Arabidopsis sp. |
66400 | - |
x * 66400, calculated from the nucleotide sequence | Zea mays |
141000 | - |
gel filtration | Arabidopsis sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + NADH | Escherichia coli | - |
5-methyltetrahydrofolate + NAD+ | - |
? | |
5,10-methylenetetrahydrofolate + NADH | Arabidopsis sp. | folate-mediated one-carbon metabolism | 5-methyltetrahydrofolate + NAD+ | - |
r | |
5,10-methylenetetrahydrofolate + NADH | Zea mays | folate-mediated one-carbon metabolism | 5-methyltetrahydrofolate + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis sp. | - |
ecotype Columbia, expression in Saccharomyces cerevisiae | - |
Escherichia coli | - |
- |
- |
Zea mays | Q9SE94 | expression in Saccharomyces cerevisiae | - |
Purification (Comment) | Organism |
---|---|
643fold purification of recombinant enzyme, expressed in Saccharomyces cerevisiae | Arabidopsis sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis sp. | - |
leaf | - |
Zea mays | - |
root | - |
Arabidopsis sp. | - |
root | - |
Zea mays | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.92 | - |
recombinant enzyme | Arabidopsis sp. |
Storage Stability | Organism |
---|---|
on ice, 3 h, loss of about 50% of activity | Arabidopsis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + NADH | - |
Escherichia coli | 5-methyltetrahydrofolate + NAD+ | - |
? | |
5,10-methylenetetrahydrofolate + NADH | folate-mediated one-carbon metabolism | Arabidopsis sp. | 5-methyltetrahydrofolate + NAD+ | - |
r | |
5,10-methylenetetrahydrofolate + NADH | folate-mediated one-carbon metabolism | Zea mays | 5-methyltetrahydrofolate + NAD+ | - |
r | |
5,10-methylenetetrahydrofolate + reduced acceptor | reverse reaction: menadione as electron acceptor | Escherichia coli | 5-methyltetrahydrofolate + oxidized acceptor | - |
r | |
5,10-methylenetetrahydrofolate + reduced acceptor | reverse reaction: menadione as electron acceptor | Arabidopsis sp. | 5-methyltetrahydrofolate + oxidized acceptor | - |
r | |
5,10-methylenetetrahydrofolate + reduced acceptor | reverse reaction: menadione as electron acceptor | Zea mays | 5-methyltetrahydrofolate + oxidized acceptor | - |
r | |
5,10-methylenetetrahydrofolate + reduced acceptor | prefers NADH as reductant | Escherichia coli | 5-methyltetrahydrofolate + oxidized acceptor | - |
r | |
5,10-methylenetetrahydrofolate + reduced acceptor | forward reaction: strong preference of NADH over NADPH | Arabidopsis sp. | 5-methyltetrahydrofolate + oxidized acceptor | - |
r | |
5,10-methylenetetrahydrofolate + reduced acceptor | forward reaction: strong preference of NADH over NADPH | Zea mays | 5-methyltetrahydrofolate + oxidized acceptor | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 66400, calculated from the nucleotide sequence | Zea mays |
dimer | 2 * 66000, SDS-PAGE | Arabidopsis sp. |
homotetramer | 4 * 33000 | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
37 | - |
assay at | Arabidopsis sp. |
37 | - |
assay at | Zea mays |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Escherichia coli | |
NADH | strong preference of NADH over NADPH as reductant | Arabidopsis sp. | |
NADH | strong preference of NADH over NADPH as reductant | Zea mays |