Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
binary complexes of PYCR1 with NADPH or proline, at 1.9 A resolution, and a ternary complex containing NADPH and a P5C/proline analog, to 1.85 A resolution. NADPH is bound to the Rossmann fold. Structures provide a model of the Michaelis complex formed during hydride transfer | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
T238A | mutation in conserved residue, about 10fold decrease in catalytic efficiency | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
NADH | wild-type, pH 7.5, 37°C | Homo sapiens | |
0.159 | - |
NADPH | mutant T238A, pH 7.5, 37°C | Homo sapiens | |
0.283 | - |
NADPH | wild-type, pH 7.5, 37°C | Homo sapiens | |
0.667 | - |
1-pyrroline-5-carboxylate | cosubstrate NADPH, wild-type, pH 7.5, 37°C | Homo sapiens | |
2.887 | - |
1-pyrroline-5-carboxylate | mutant T238A, wild-type, pH 7.5, 37°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P32322 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-pyrroline-5-carboxylate + NADH + H+ | - |
Homo sapiens | L-proline + NAD+ | - |
? | |
1-pyrroline-5-carboxylate + NADPH + H+ | - |
Homo sapiens | L-proline + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | enzyme forms a concentration-dependent decamer in solution, sedimentation velocity data | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
Pycr1 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14 | - |
1-pyrroline-5-carboxylate | mutant T238A, wild-type, pH 7.5, 37°C | Homo sapiens | |
23 | - |
NADPH | mutant T238A, pH 7.5, 37°C | Homo sapiens | |
31 | - |
1-pyrroline-5-carboxylate | cosubstrate NADPH, wild-type, pH 7.5, 37°C | Homo sapiens | |
74 | - |
NADPH | wild-type, pH 7.5, 37°C | Homo sapiens | |
218 | - |
NADH | wild-type, pH 7.5, 37°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
50 | - |
1-pyrroline-5-carboxylate | mutant T238A, wild-type, pH 7.5, 37°C | Homo sapiens | |
260 | - |
NADPH | wild-type, pH 7.5, 37°C | Homo sapiens | |
470 | - |
1-pyrroline-5-carboxylate | cosubstrate NADPH, wild-type, pH 7.5, 37°C | Homo sapiens | |
1400 | - |
NADPH | mutant T238A, pH 7.5, 37°C | Homo sapiens | |
3100 | - |
NADH | wild-type, pH 7.5, 37°C | Homo sapiens |