Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) pLysS cells | Streptococcus pyogenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Streptococcus pyogenes | |
additional information | not inhibited by NADPH | Streptococcus pyogenes | |
NADH | slight inhibition with 25% reduction of the catalytic rate at 200 mM | Streptococcus pyogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
NADPH | apparent value, at pH 7.5 and 37°C | Streptococcus pyogenes | |
0.176 | - |
NADH | apparent value, at pH 7.5 and 37°C | Streptococcus pyogenes | |
0.184 | - |
1-pyrroline-5-carboxylate | apparent value, with NADPH as cosubstrate, at pH 7.5 and 37°C | Streptococcus pyogenes | |
0.349 | - |
1-pyrroline-5-carboxylate | apparent value, with NADH as cosubstrate, at pH 7.5 and 37°C | Streptococcus pyogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pyogenes | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-pyrroline-5-carboxylate + NADH + H+ | - |
Streptococcus pyogenes | L-proline + NAD+ | - |
? | |
1-pyrroline-5-carboxylate + NADPH + H+ | - |
Streptococcus pyogenes | L-proline + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DELTA1-pyrroline-5-carboxylate reductase | - |
Streptococcus pyogenes |
P5CR | - |
Streptococcus pyogenes |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
340 | - |
NADPH | at pH 7.5 and 37°C | Streptococcus pyogenes | |
620 | - |
NADH | at pH 7.5 and 37°C | Streptococcus pyogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | 7.8 | - |
Streptococcus pyogenes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | with NADH the activity is markedly inhibited by physiological levels of NADP+, so NADH cannot serve as electron donor in vivo | Streptococcus pyogenes | |
NADPH | - |
Streptococcus pyogenes |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
29 | - |
at pH 7.5 and 37°C | Streptococcus pyogenes | EDTA |