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Literature summary for 1.5.1.2 extracted from

  • Rena, A.B.; Splittstoesser, W.E.
    Proline dehydrogenase and pyrroline-5-carboxylate reductase from pumpkin cotylenons (1975), Phytochemistry, 14, 657-661.
No PubMed abstract available
Search for more literature for 1.5.1.2

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
 Cucurbita moschata
Ag+ 44% inactivation at 0.7 mM Cucurbita moschata
ATP
-
 Cucurbita moschata
Cu2+ not Cucurbita moschata
cysteine 77% inactivation at 10 mM Cucurbita moschata
Hg2+
-
 Cucurbita moschata
iodoacetate 10% inactivation at 1 mM Cucurbita moschata
NaHSO3 88% inactivation at 1 mM Cucurbita moschata
NH2OH 7% inactivation at 1 mM Cucurbita moschata
p-chloromercuribenzoate not Cucurbita moschata
proline
-
 Cucurbita moschata
Sodium bisulfite
-
 Cucurbita moschata
Zn2+ 14% inactivation at 1 mM Cucurbita moschata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 gel filtration Cucurbita moschata

Organism

Organism UniProt Comment Textmining
Cucurbita moschata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
copurification with proline oxidase Cucurbita moschata

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
 Cucurbita moschata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2070
-
-
 Cucurbita moschata

Storage Stability

Storage Stability Organism
3°C, 0.1 M sodium phosphate buffer pH 7.6, 10% (NH4)2SO4, 7 days Cucurbita moschata

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 15 min stable Cucurbita moschata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 pyrroline-5-carboxylate reductase activity Cucurbita moschata
9.8 10.4 proline dehydrogenase activity Cucurbita moschata

pH Range

pH Minimum pH Maximum Comment Organism
4 8 pyrroline-5-carboxylate reductase activity Cucurbita moschata
9.2 10.5 proline dehydrogenase activity Cucurbita moschata