Any feedback?
Literature summary for 1.5.1.2 extracted from
- Partial purification and some properties of delta1-pyrroline-5-carboxylate reductase from Escherichia coli (1977), J. Bacteriol., 129, 108-114.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NADH | above 0.16 mM | Escherichia coli |  |
| NADP+ | - |
Escherichia coli | |
| NADPH | above 0.13 mM | Escherichia coli | |
| p-chloromercuribenzoate | complete inhibition at 0.125 mM, can be prevented by addition of dithiothreitol | Escherichia coli | |
| proline | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.14 | - |
1-pyrroline-5-carboxylate | with NADH | Escherichia coli | |
| 0.15 | - |
1-pyrroline-5-carboxylate | with NADPH | Escherichia coli | |
| 0.23 | - |
NADH | NADH | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 320000 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
K-12 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 41.25 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-pyrroline-5-carboxylate + NAD(P)H + H+ | specific for L-isomer | Escherichia coli | L-proline + NAD(P)+ | - |
ir | |
| 1-pyrroline-5-carboxylate + NADH + H+ | - |
Escherichia coli | L-proline + NAD+ | - |
r | |
| 1-pyrroline-5-carboxylate + NADPH + H+ | - |
Escherichia coli | L-proline + NADP+ | - |
r | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 40 | - |
Escherichia coli |
| 60 | - |
inactivation above | Escherichia coli |
| 67 | - |
50% inactivation after 5 min | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | preferentially | Escherichia coli | |
| NADPH | preferentially | Escherichia coli | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
