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Literature summary for 1.5.1.2 extracted from
- Purification and characterization of pyrroline-5-carboxylate reductase from bovine retina (1982), Biochim. Biophys. Acta, 717, 215-219.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | competitive with respect to NADH, 50% inhibition at 0.1 mM | Bos taurus |  |
| proline | 39% inhibition of the NADH dependent activity and 17% of the NADPH dependent activity at 10 mM | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0071 | - |
NADPH | - |
Bos taurus | |
| 0.125 | - |
1-pyrroline-5-carboxylate | - |
Bos taurus | |
| 0.2 | - |
NADH | - |
Bos taurus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | 2fold increase at 70 mM | Bos taurus | |
| phosphate | 10 mM, reaction enhancement | Bos taurus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| retina | distribution in bovine cornea and lens | Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
subcellular distribution | Bos taurus |
| 4.167 | - |
- |
Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, inactivation in few days, unstable in the absence of NADPH | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-pyrroline-5-carboxylate + NADH + H+ | - |
Bos taurus | L-proline + NAD+ | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | - |
Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Bos taurus | |
| NADPH | - |
Bos taurus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
ATP | - |
Bos taurus | |
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Release 2023.1 (January 2023)
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