Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
up to 480000 kDa, quarternary structure changes drastically with buffer environment | Hordeum vulgare |
30000 | - |
- |
Hordeum vulgare |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | - |
barley | - |
Purification (Comment) | Organism |
---|---|
homogeneity | Hordeum vulgare |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Hordeum vulgare | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
507 | - |
- |
Hordeum vulgare |
Storage Stability | Organism |
---|---|
-80°C, 5% glycerol, 2 mM dithiothreitol, 1 month | Hordeum vulgare |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-pyrroline-5-carboxylate + NAD(P)H + H+ | - |
Hordeum vulgare | L-proline + NAD(P)+ | - |
? | |
L-proline benzyl ester + NAD+ | 24% of activity with proline | Hordeum vulgare | L-pyrroline-5-carboxy benzyl ester + NADH | - |
? | |
L-proline methyl ester + NAD+ | 16% of activity with proline | Hordeum vulgare | 1-pyrroline-5-carboxy methyl ester + NADH | - |
? | |
L-proline t-butyl ester + NAD+ | 10% of activity with proline | Hordeum vulgare | 1-pyrroline-5-carboxy tert-butylester + NADH | - |
? | |
additional information | not: D-proline, L-hydroxyproline, L-azatidine-2-carboxylate, no other amino acids | Hordeum vulgare | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
polymer | 12-16 * 30000 SDS-PAGE | Hordeum vulgare |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1270 | - |
pyrroline-5-carboxylate | - |
Hordeum vulgare |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | 7.5 | pyrroline-5-carboxylate reductase activity | Hordeum vulgare |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
no proline dehydrogenase activity below pH 8.5 | Hordeum vulgare |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Hordeum vulgare | |
NADPH | - |
Hordeum vulgare |