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Literature summary for 1.5.1.2 extracted from
- Pyrroline-5-carboxylate reductase is in pea (Pisum sativum L.) leaf chloroplasts (1989), Plant Physiol., 91, 581-586.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Pisum sativum |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
NADPH | etiolated shoots | Pisum sativum | |
| 0.12 | - |
NADPH | chloroplast | Pisum sativum | |
| 0.19 | - |
NADH | chloroplast | Pisum sativum | |
| 0.43 | - |
NADH | etiolated shoots | Pisum sativum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Pisum sativum | 9507 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| ammonium acetate | increase of activity | Pisum sativum | |
| KCl | increase of activity at 100 mM | Pisum sativum | |
| Mg2+ | increase of activity at 10 mM | Pisum sativum | |
| NH4Cl | increase of activity | Pisum sativum | |
| potassium acetate | increase of activity | Pisum sativum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyrroline-5-carboxylate + NAD(P)H + H+ | Pisum sativum | final reaction of proline synthesis | L-proline + NAD(P)+ | - |
? | |
| pyrroline-5-carboxylate + NAD(P)H + H+ | Pisum sativum | discussion of role in vivo | L-proline + NAD(P)+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | from etiolated shoots | Pisum sativum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.042 | - |
- |
Pisum sativum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-pyrroline-5-carboxylate + NADH + H+ | - |
Pisum sativum | L-proline + NAD+ | - |
r | |
| pyrroline-5-carboxylate + NAD(P)H + H+ | final reaction of proline synthesis | Pisum sativum | L-proline + NAD(P)+ | - |
? | |
| pyrroline-5-carboxylate + NAD(P)H + H+ | discussion of role in vivo | Pisum sativum | L-proline + NAD(P)+ | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
in choroplasts | Pisum sativum |
| 7.5 | - |
in etiolated shoots | Pisum sativum |
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