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Literature summary for 1.5.1.10 extracted from

  • Vashishtha, A.K.; West, A.H.; Cook, P.F.
    Overall kinetic mechanism of saccharopine dehydrogenase (L-glutamate forming) from Saccharomyces cerevisiae (2008), Biochemistry, 47, 5417-5423.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21/(DE-3) RIL cells Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
2-amino-6-heptenoic acid competitive inhibition versus L-alpha-aminoadipate-delta-semialdehyde, uncompetitive inhibition versus NADPH, and noncompetitive inhibitition versus L-glutamate Saccharomyces cerevisiae
2-oxoglutarate competitive inhibition versus L-glutamate and uncompetitive inhibition versus L-alpha-aminoadipate-delta-semialdehyde and NADPH; competitive inhibition versus saccharopine and uncompetitive inhibition versus NADP+ Saccharomyces cerevisiae
alpha-AASA shows noncompetitive inhibition versus saccharopine and uncompetitive inhibition versus NADP+ Saccharomyces cerevisiae
glyoxylic acid competitive inhibition versus saccharopine and uncompetitive inhibition versus NADP+ Saccharomyces cerevisiae
L-glutamate exhibits noncompetitive inhibition versus NADP+ and saccharopine Saccharomyces cerevisiae
L-leucine competitive inhibition versus saccharopine and uncompetitive inhibition versus NADP+ Saccharomyces cerevisiae
L-ornithine competitive inhibition versus saccharopine and uncompetitive inhibition versus NADP+ Saccharomyces cerevisiae
L-pipecolic acid competitive inhibition versus saccharopine and uncompetitive inhibition versus NADP+ Saccharomyces cerevisiae
N-oxalylglycine competitive inhibition versus saccharopine and uncompetitive inhibition versus NADP+ Saccharomyces cerevisiae
NADP+ competitive inhibition versus NADPH, noncompetitive inhibition versus L-alpha-aminoadipate-delta-semialdehyde and L-glutamate Saccharomyces cerevisiae
NADPH inhibition is competitive versus NADP+ and noncompetitive versus saccharopine Saccharomyces cerevisiae
saccharopine exhibits noncompetitive inhibition against L-alpha-aminoadipate-delta-semialdehyde, L-glutamate, and NADPH Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminoadipate 6-semialdehyde + L-glutamate + NADPH + H+
-
Saccharomyces cerevisiae N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
r

Synonyms

Synonyms Comment Organism
saccharopine dehydrogenase (L-glutamate forming)
-
Saccharomyces cerevisiae
saccharopine reductase
-
Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Saccharomyces cerevisiae