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Literature summary for 1.5.1.10 extracted from

  • Storts, D.R.; Bhattacharjee, J.K.
    Purification and properties of saccharopine dehydrogenase (glutamate forming) in the Saccharomyces cerevisiae lysine biosynthetic pathway (1987), J. Bacteriol., 169, 416-418.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.5.1.10

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Saccharomyces cerevisiae
2,2'-bipyridine
-
 Saccharomyces cerevisiae
HgCl2
-
 Saccharomyces cerevisiae
additional information not: carbonyl reagents Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.054
-
 NAD+
-
 Saccharomyces cerevisiae
2.32
-
 L-saccharopine
-
 Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 1 * 50000, SDS-PAGE Saccharomyces cerevisiae
67000
-
 gel filtration with Sephadex G-100 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-aminoadipate 6-semialdehyde + L-glutamate + NADPH Saccharomyces cerevisiae lysine biosynthesis N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 baker's yeast
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no glycoprotein
-
 Saccharomyces cerevisiae

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.24
-
 crude extract Saccharomyces cerevisiae
269.4
-
 after purification Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-70°C, pH 8.0, 10 mM 2-mercaptoethanol, 5 mM EDTA Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminoadipate 6-semialdehyde + L-glutamate + NADPH
-
 Saccharomyces cerevisiae N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine r
2-aminoadipate 6-semialdehyde + L-glutamate + NADPH lysine biosynthesis Saccharomyces cerevisiae N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O
-
 Saccharomyces cerevisiae L-glutamate + (S)-2-amino-6-oxohexanoate + NADH + H+
-
 r

Subunits

Subunits Comment Organism
monomer 1 * 50000, SDS-PAGE Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
34
-
 denaturation above Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
 saccharopine degradation Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
9 10 about 50% of activity maximum at pH 9 and 10 Saccharomyces cerevisiae