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Literature summary for 1.4.9.1 extracted from

  • Fujieda, N.; Mori, M.; Ikeda, T.; Kano, K.
    The silent form of quinohemoprotein amine dehydrogenase from Paracoccus denitrificans (2009), Biosci. Biotechnol. Biochem., 73, 524-529.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
benzylamine activates sQH-AmDH Paracoccus denitrificans
Dithionite rapidly activates sQH-AmDH, activation process involves a reduction process Paracoccus denitrificans
dithiothreitol rapidly activates sQH-AmDH, activation process involves a reduction process Paracoccus denitrificans
n-butylamine activates sQH-AmDH Paracoccus denitrificans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36500
-
SDS-PAGE, heme 2 shows two bands Paracoccus denitrificans
59500
-
SDS-PAGE, heme 2 shows two bands Paracoccus denitrificans

Organism

Organism UniProt Comment Textmining
Paracoccus denitrificans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ion-exchange chromatography with DEAE-Toyopearl resin Paracoccus denitrificans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
heme 2 does not show any enzymatic activity for amine oxidation. Heme 2 is slowly activated in 10-30 h during incubation with n-butylamine. Enzyme is faster activated with benzylamine Paracoccus denitrificans
additional information
-
sQH-AmDH is not electrochemically activated by direct electrolytic reduction with a column electrode at -0.5 V, but it is electrochemically activated by mediated electrolytic reduction with a column electrode Paracoccus denitrificans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzylamine + H2O + ferricyanide
-
Paracoccus denitrificans benzaldehyde + NH3 + reduced ferricyanide
-
?
n-butylamine + H2O + ferricyanide
-
Paracoccus denitrificans butanal + NH3 + reduced ferricyanide
-
?

Subunits

Subunits Comment Organism
heterotrimer gel filtration, mass spectroscopy and UV-VIS spectroscopy: the gamma subunit of sQH-AmDH shows a sharp peak at 390 nm in UV-VIS spectrum clearly distinguishable from that of QH-AmDH. Electrospray ionization mass spectrometric analysis shows that the molecular mass of the gamma subunit of sQH-AmDH is larger than that of QH-AmDH by 15.6. The data suggest that the cysteine tryptophylquinone-like moiety (catalytic center) of the gamma subunit of sQH-AmDH is an oxime Paracoccus denitrificans

Synonyms

Synonyms Comment Organism
Heme 2 inactive form of QH-AmDH Paracoccus denitrificans
quinohemoprotein amine dehydrogenase
-
Paracoccus denitrificans
sQH-AmDH silent form of QH-AmDH Paracoccus denitrificans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Paracoccus denitrificans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Paracoccus denitrificans