Cloned (Comment) | Organism |
---|---|
Expression in Escherichia coli, the production of knock out-mutants unable to catabolize 2-phenylethylamine is carried out by mutagenesis. | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | enzyme contains two heme groups | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
8579 | - |
calculated gamma-subunit | Pseudomonas putida |
39360 | - |
calculated for beta-subunit, containing a 26 aa sequence which seems to correspond to a signal peptide that might be also involved in the correct translocation from the bacterial cytoplasm to the periplasmic space. | Pseudomonas putida |
54230 | - |
calculated for alpha-subunit, existence in this protein of two consensus sequences (CXXCH) identifying as binding pockets for the two haem groups | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phenylethylamine + 2 H2O + 2 acceptor | Pseudomonas putida | primary amine | 2-phenylacetic acid + NH3 + 2 reduced acceptor | - |
? | |
additional information | Pseudomonas putida | an essential enzyme for the aerobic degradation of many primary amines even though they have quite different chemical structures (aromatic or aliphatic) | ? | - |
? | |
additional information | Pseudomonas putida | when different QHNDH mutants (peaA, peaC and peaD) are transformed with a genetic construction containing the peaABCD cluster, all the recombinant strains efficiently catabolized 2-phenylethylamine as well as other primary amines like propyl-, butyl- and pentylamine. | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Pseudomonas putida | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phenylethylamine + 2 H2O + 2 acceptor | primary amine | Pseudomonas putida | 2-phenylacetic acid + NH3 + 2 reduced acceptor | - |
? | |
additional information | an essential enzyme for the aerobic degradation of many primary amines even though they have quite different chemical structures (aromatic or aliphatic) | Pseudomonas putida | ? | - |
? | |
additional information | when different QHNDH mutants (peaA, peaC and peaD) are transformed with a genetic construction containing the peaABCD cluster, all the recombinant strains efficiently catabolized 2-phenylethylamine as well as other primary amines like propyl-, butyl- and pentylamine. | Pseudomonas putida | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotrimer | determinated by in silico-experiments, beta-subunit with 349 aa, gamma-subunit with 79 aa and an alpha-subunit with 494 aa | Pseudomonas putida |
More | protein PeaB, although not interacting with the active QHNDH along the catalytic reaction, is needed for the correct functionality of this enzyme and for the post-translational modification of the gamma-subunit before its translocation to the periplasmic space. | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
amine dehydrogenase | - |
Pseudomonas putida |
QH-AmDH | - |
Pseudomonas putida |
QHNDH | - |
Pseudomonas putida |
quinohaemoprotein amine dehydrogenase | - |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cysteine tryptophylquinone | This enzyme contains an unusual redox cofactor (CTQ) and two hemes acting as redox active groups | Pseudomonas putida |