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Literature summary for 1.4.9.1 extracted from

  • Wang, Y.; Sun, D.; Davidson, V.L.
    Use of indirect site-directed mutagenesis to alter the substrate specificity of methylamine dehydrogenase (2002), J. Biol. Chem., 277, 4119-4122.
    View publication on PubMed
Search for more literature for 1.4.9.1

Protein Variants

Protein Variants Comment Organism
alphaF55A 1656fold increase in Km-value for methylamine compared to wild-type enzyme, 484fold increase in Km-value for ethylamine compared to wild-type enzyme, 36fold increase in Km-value for propylamine compared to wild-type enzyme, 3.6fold decrease in Km-value for butylamine compared to wild-type enzyme, 53.2fold decrease in Km-value for 1-aminopentane compared to wild-type enzyme, 34.3fold decrease in Km-value for 1-6-diaminohexane compared to wild-type enzyme, 54.3fold decrease in Km-value for 1,7-diaminoheptane compared to wild-type enzyme Paracoccus denitrificans
alphaF55I 1.2fold decrease in Km-value for methylamine compared to wild-type enzyme, 18.9fold increase in Km-value for ethylamine compared to wild-type enzyme, 5.6fold increase in Km-value for propylamine compared to wild-type enzyme, 4.2fold decrease in Km-value for butylamine compared to wild-type enzyme, 10fold decrease in Km-value for 1-aminopentane compared to wild-type enzyme, 7.3fold decrease in Km-value for 1-6-diaminohexane compared to wild-type enzyme, 6.7fold decrease in Km-value for 1,7-diaminoheptane compared to wild-type enzym. Ability to discriminate between amines of different chain length is abolished Paracoccus denitrificans
betaI107N 27.8fold increase in Km-value for methylamine compared to wild-type enzyme, 44.2fold increase in Km-value for ethylamine compared to wild-type enzyme, 8.5fold decrease in Km-value for propylamine compared to wild-type enzyme, 124fold decrease in Km-value for butylamine compared to wild-type enzyme, 62.5fold decrease in Km-value for 1-aminopentane compared to wild-type enzyme, 23.2fold decrease in Km-value for 1-6-diaminohexane compared to wild-type enzyme, 5.6fold decrease in Km-value for 1,7-diaminoheptane compared to wild-type enzyme. Mutant enzyme exhibity a strong preference for 1-aminopentane compared to strong preference for methylamine of the wild-type enzyme Paracoccus denitrificans
betaI107V mutant enzyme exhibits a strong preference for propylamine compared to strong preference for methylamine of the wild-type enzyme. 7.7fold increase in Km-value for methylamine compared to wild-type enzyme, 17.9fold increase in Km-value for ethylamine compared to wild-type enzyme, 6fold decrease in Km-value for propylamine compared to wild-type enzyme, 9.9fold decrease in Km-value for butylamine compared to wild-type enzyme, 19.2fold decrease in Km-value for 1-aminopentane compared to wild-type enzyme, 4.2fold decrease in Km-value for 1-6-diaminohexane compared to wild-type enzyme, 1.3fold decrease in Km-value for 1,7-diaminoheptane compared to wild-type enzyme Paracoccus denitrificans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 1-aminopentane pH 7.5, 30°C, mutant enzyme betaI107N Paracoccus denitrificans
0.006
-
 Propylamine pH 7.5, 30°C, mutant enzyme betaI107V Paracoccus denitrificans
0.007
-
 1,7-Diaminoheptane pH 7.5, 30°C, mutant enzyme alphaF55A Paracoccus denitrificans
0.007
-
 Butylamine pH 7.5, 30°C, mutant enzyme betaI107N Paracoccus denitrificans
0.008
-
 Propylamine pH 7.5, 30°C, mutant enzyme betaI107N Paracoccus denitrificans
0.009
-
 methylamine pH 7.5, 30°C, wild-type enzyme Paracoccus denitrificans
0.019
-
 ethylamine pH 7.5, 30°C, wild-type enzyme Paracoccus denitrificans
0.021
-
 1,6-diaminohexane pH 7.5, 30°C, mutant enzyme alphaF55A Paracoccus denitrificans
0.031
-
 1,6-diaminohexane pH 7.5, 30°C, mutant enzyme betaI107N Paracoccus denitrificans
0.036
-
 Propylamine pH 7.5, 30°C, wild-type enzyme Paracoccus denitrificans
0.047
-
 1-aminopentane pH 7.5, 30°C, mutant enzyme alphaF55A Paracoccus denitrificans
0.057
-
 1,7-Diaminoheptane pH 7.5, 30°C, mutant enzyme alphaF55I Paracoccus denitrificans
0.06
-
 methylamine pH 7.5, 30°C, mutant enzyme alphaF55I Paracoccus denitrificans
0.068
-
 1,7-Diaminoheptane pH 7.5, 30°C, mutant enzyme betaI107N Paracoccus denitrificans
0.069
-
 methylamine pH 7.5, 30°C, mutant enzyme betaI107V Paracoccus denitrificans
0.088
-
 Butylamine pH 7.5, 30°C, mutant enzyme betaI107V Paracoccus denitrificans
0.099
-
 1,6-diaminohexane pH 7.5, 30°C, mutant enzyme alphaF55I Paracoccus denitrificans
0.13
-
 1-aminopentane pH 7.5, 30°C, mutant enzyme betaI107V Paracoccus denitrificans
0.17
-
 1,6-diaminohexane pH 7.5, 30°C, mutant enzyme betaI107V Paracoccus denitrificans
0.2
-
 Propylamine pH 7.5, 30°C, mutant enzyme alphaF55I Paracoccus denitrificans
0.24
-
 Butylamine pH 7.5, 30°C, mutant enzyme alphaF55A Paracoccus denitrificans
0.25
-
 1-aminopentane pH 7.5, 30°C, mutant enzyme alphaF55I Paracoccus denitrificans
0.25
-
 methylamine pH 7.5, 30°C, mutant enzyme betaI107N Paracoccus denitrificans
0.29
-
 1,7-Diaminoheptane pH 7.5, 30°C, mutant enzyme betaI107V Paracoccus denitrificans
0.34
-
 ethylamine pH 7.5, 30°C, mutant enzyme betaI107V Paracoccus denitrificans
0.36
-
 ethylamine pH 7.5, 30°C, mutant enzyme alphaF55I Paracoccus denitrificans
0.37
-
 Butylamine pH 7.5, 30°C, mutant enzyme alphaF55I Paracoccus denitrificans
0.38
-
 1,7-Diaminoheptane pH 7.5, 30°C, wild-type enzyme Paracoccus denitrificans
0.72
-
 1,6-diaminohexane pH 7.5, 30°C, wild-type enzyme Paracoccus denitrificans
0.84
-
 ethylamine pH 7.5, 30°C, mutant enzyme betaI107N Paracoccus denitrificans
0.87
-
 Butylamine pH 7.5, 30°C, wild-type enzyme Paracoccus denitrificans
1.3
-
 Propylamine pH 7.5, 30°C, mutant enzyme alphaF55A Paracoccus denitrificans
2.5
-
 1-aminopentane pH 7.5, 30°C, wild-type enzyme Paracoccus denitrificans
9.2
-
 ethylamine pH 7.5, 30°C, mutant enzyme alphaF55A Paracoccus denitrificans
14.9
-
 methylamine pH 7.5, 30°C, mutant enzyme alphaF55A Paracoccus denitrificans

Organism

Organism UniProt Comment Textmining
Paracoccus denitrificans
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,6-diaminohexane + acceptor + H2O acceptor: phenazine ethosulfate/2,6-dichlorophenolindophenol Paracoccus denitrificans ?
-
 ?
1,7-diaminoheptane + acceptor + H2O acceptor: phenazine ethosulfate/2,6-dichlorophenolindophenol Paracoccus denitrificans ?
-
 ?
1-aminopentane + acceptor + H2O acceptor: phenazine ethosulfate/2,6-dichlorophenolindophenol Paracoccus denitrificans pentanal + NH3 + reduced acceptor
-
 ?
butylamine + acceptor + H2O acceptor: phenazine ethosulfate/2,6-dichlorophenolindophenol Paracoccus denitrificans butanal + NH3 + reduced acceptor
-
 ?
ethylamine + acceptor + H2O acceptor: phenazine ethosulfate/2,6-dichlorophenolindophenol Paracoccus denitrificans acetaldehyde + NH3 + reduced acceptor
-
 ?
methylamine + acceptor + H2O acceptor: phenazine ethosulfate/2,6-dichlorophenolindophenol Paracoccus denitrificans formaldehyde + NH3 + reduced acceptor
-
 ?
propylamine + acceptor + H2O acceptor: phenazine ethosulfate/2,6-dichlorophenolindophenol Paracoccus denitrificans propionaldehyde + NH3 + reduced acceptor
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2
-
 methylamine pH 7.5, 30°C, mutant enzyme alphaF55I Paracoccus denitrificans
3.1
-
 Propylamine pH 7.5, 30°C, mutant enzyme betaI107V Paracoccus denitrificans
3.4
-
 1,6-diaminohexane pH 7.5, 30°C, mutant enzyme alphaF55I Paracoccus denitrificans
3.4
-
 1,7-Diaminoheptane pH 7.5, 30°C, mutant enzyme alphaF55I Paracoccus denitrificans
3.4
-
 1-aminopentane pH 7.5, 30°C, mutant enzyme betaI107V Paracoccus denitrificans
4.1
-
 Propylamine pH 7.5, 30°C, mutant enzyme betaI107N Paracoccus denitrificans
4.2
-
 Butylamine pH 7.5, 30°C, mutant enzyme betaI107N Paracoccus denitrificans
4.2
-
 1-aminopentane pH 7.5, 30°C, mutant enzyme betaI107N Paracoccus denitrificans
4.5
-
 ethylamine pH 7.5, 30°C, mutant enzyme betaI107N Paracoccus denitrificans
5.2
-
 Propylamine pH 7.5, 30°C, mutant enzyme alphaF55I Paracoccus denitrificans
5.4
-
 Butylamine pH 7.5, 30°C, mutant enzyme alphaF55I Paracoccus denitrificans
5.4
-
 1-aminopentane pH 7.5, 30°C, mutant enzyme alphaF55I Paracoccus denitrificans
6.2
-
 ethylamine pH 7.5, 30°C, mutant enzyme betaI107V Paracoccus denitrificans
14
-
 Butylamine pH 7.5, 30°C, mutant enzyme betaI107V Paracoccus denitrificans
15
-
 ethylamine pH 7.5, 30°C, mutant enzyme alphaF55I Paracoccus denitrificans
15
-
 1,7-Diaminoheptane pH 7.5, 30°C, mutant enzyme betaI107N Paracoccus denitrificans
16
-
 1,6-diaminohexane pH 7.5, 30°C, mutant enzyme betaI107N Paracoccus denitrificans
17
-
 1,6-diaminohexane pH 7.5, 30°C, wild-type enzyme Paracoccus denitrificans
17
-
 1-aminopentane pH 7.5, 30°C, wild-type enzyme Paracoccus denitrificans
19
-
 1,7-Diaminoheptane pH 7.5, 30°C, mutant enzyme betaI107V Paracoccus denitrificans
20
-
 1-aminopentane pH 7.5, 30°C, mutant enzyme alphaF55A Paracoccus denitrificans
20
-
 methylamine pH 7.5, 30°C, mutant enzyme betaI107V Paracoccus denitrificans
22
-
 Butylamine pH 7.5, 30°C, wild-type enzyme Paracoccus denitrificans
23
-
 ethylamine pH 7.5, 30°C, mutant enzyme alphaF55A Paracoccus denitrificans
24
-
 Propylamine pH 7.5, 30°C, mutant enzyme alphaF55A Paracoccus denitrificans
24
-
 ethylamine pH 7.5, 30°C, wild-type enzyme Paracoccus denitrificans
26
-
 1,6-diaminohexane pH 7.5, 30°C, mutant enzyme betaI107V Paracoccus denitrificans
27
-
 Propylamine pH 7.5, 30°C, wild-type enzyme Paracoccus denitrificans
27
-
 1,7-Diaminoheptane pH 7.5, 30°C, wild-type enzyme Paracoccus denitrificans
30
-
 methylamine pH 7.5, 30°C, wild-type enzyme Paracoccus denitrificans
32
-
 1,7-Diaminoheptane pH 7.5, 30°C, mutant enzyme alphaF55A Paracoccus denitrificans
34
-
 Butylamine pH 7.5, 30°C, mutant enzyme alphaF55A Paracoccus denitrificans
34
-
 methylamine pH 7.5, 30°C, mutant enzyme betaI107N Paracoccus denitrificans
43
-
 1,6-diaminohexane pH 7.5, 30°C, mutant enzyme alphaF55A Paracoccus denitrificans
77
-
 methylamine pH 7.5, 30°C, mutant enzyme alphaF55A Paracoccus denitrificans