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Literature summary for 1.4.9.1 extracted from
- Purification and characterization of a heme-containing amine dehydrogenase from Pseudomonas putida (1978), J. Bacteriol., 134, 837-843.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 8-hydroxyquinoline | slight | Pseudomonas putida |  |
| Cuprizone | - |
Pseudomonas putida | |
| hydroxylamine | - |
Pseudomonas putida | |
| iodoacetate | - |
Pseudomonas putida | |
| isoniazid | - |
Pseudomonas putida | |
| KCN | - |
Pseudomonas putida | |
| p-chloromercuribenzoate | - |
Pseudomonas putida | |
| Quinacrine | - |
Pseudomonas putida | |
| Semicarbazide | - |
Pseudomonas putida | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | no effect | Pseudomonas putida | |
| Co2+ | slight | Pseudomonas putida | |
| Mn2+ | - |
Pseudomonas putida | |
| Na+ | no effect | Pseudomonas putida | |
| Zn2+ | - |
Pseudomonas putida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
1 * 58000 + 1 * 42000, SDS-PAGE | Pseudomonas putida |
| 58000 | - |
1 * 58000 + 1 * 42000, SDS-PAGE | Pseudomonas putida |
| 95300 | - |
sedimentation equilibrium | Pseudomonas putida |
| 112000 | - |
gel filtration | Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas putida |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.67 | - |
- |
Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | broad specificity | Pseudomonas putida | ? | - |
? | |
| additional information | little or no activity with isoamines, L-ornithine, L-lysine and certain diamines or polyamines | Pseudomonas putida | ? | - |
? | |
| RCH2NH2 + acceptor + H2O | cytochrome c or artificial electron acceptor | Pseudomonas putida | RCHO + NH3 + reduced acceptor | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 58000 + 1 * 42000, SDS-PAGE | Pseudomonas putida |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme c | hemoprotein which contains 2.01 mol per mol of enzyme | Pseudomonas putida | |
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