Literature summary for 1.4.3.5 extracted from

Biswal, B.K.; Cherney, M.M.; Wang, M.; Garen, C.; James, M.N.
Structures of Mycobacterium tuberculosis pyridoxine 5'-phosphate oxidase and its complexes with flavin mononucleotide and pyridoxal 5'-phosphate (2005), Acta Crystallogr. Sect. D, 61, 1492-1499.

Cloned(Commentary)

Cloned (Comment)	Organism
expression of the GST-tagged enzyme from strain Rv1155 in Escherichia coli strain BL21(DE3)	Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme from strain Rv1155, sitting drop vapour diffusion method, 9 mg/ml enzyme in 50 mM Tris-HCl, pH 8.0, 25-50 mM KF, and 10 mM glutathione, mixed with an equal volume of precipitation solution containing 0.1 M HEPES, pH 7.5, 8% ethylene glycol, and 10% PEG 8000, macroseeding with hanging drops containing equal volumes of protein and precipitation solutions overnight prior to seeding, 1-2 weeks, cryoprotection by 30% ethylene glycol, X-ray diffraction structure determination and analysis at 1.7-2.2 A, modeling	Mycobacterium tuberculosis

Crystallization (Comment)

Organism

purified recombinant enzyme from strain Rv1155, sitting drop vapour diffusion method, 9 mg/ml enzyme in 50 mM Tris-HCl, pH 8.0, 25-50 mM KF, and 10 mM glutathione, mixed with an equal volume of precipitation solution containing 0.1 M HEPES, pH 7.5, 8% ethylene glycol, and 10% PEG 8000, macroseeding with hanging drops containing equal volumes of protein and precipitation solutions overnight prior to seeding, 1-2 weeks, cryoprotection by 30% ethylene glycol, X-ray diffraction structure determination and analysis at 1.7-2.2 A, modeling

Mycobacterium tuberculosis

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	O06553	strains H37Rv and Rv1155	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant GST-tagged enzyme from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, removal of the GST-tag by rTEV protease, followed by dialysis and ultrafiltration	Mycobacterium tuberculosis

Subunits

Subunits	Comment	Organism
More	structure modeling and analysis, overview	Mycobacterium tuberculosis

Synonyms

Synonyms	Comment	Organism
pyridoxine 5'-phosphate oxidase	-	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)