Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-deoxypyridoxine 5'-phosphate | competitive inhibition | Escherichia coli | |
pyridoxal 5'-phosphate | competitive inhibition | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
pyridoxine 5'-phosphate | recombinant enzyme | Escherichia coli | |
0.105 | - |
pyridoxamine 5'-phosphate | recombinant enzyme | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
2 * 28000, SDS-PAGE | Escherichia coli |
51000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, DEAE-cellulose, hydroxyapatite, CM-cellulose, Bio-Gel | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.37 | - |
recombinant enzyme | Escherichia coli |
Storage Stability | Organism |
---|---|
-75°C, 15% glycerol, several months, no loss in activity | Escherichia coli |
4°C, several weeks, no loss of activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyridoxamine 5'-phosphate + H2O + O2 | - |
Escherichia coli | pyridoxal 5'-phosphate + NH3 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 28000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
no loss in activity after incubation at temperatures below 50°C, 30% to 40% activity loss after 15 min at 65°C | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.76 | - |
pyridoxine 5'-phosphate | recombinant enzyme | Escherichia coli | |
1.72 | - |
pyridoxamine 5'-phosphate | recombinant enzyme | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
pyridoxine phosphate oxidase activity, slight optimum | Escherichia coli |
8.5 | 10 | pyridoxamine phosphate oxidase activity remains nearly constant between pH 8.5 and 10.0 | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | pyridoxine phosphate oxidase activity | Escherichia coli |
6.5 | 10 | pyridoxamine phosphate oxidase activity is absent at pH 6.0, rises sharply from pH 6.0 to 8.5 and remains constantly high between pH 8.5 and 10.0 | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
pyridoxal 5'-phosphate | - |
Escherichia coli | |
0.105 | - |
4'-deoxypyridoxine-5'-phosphate | - |
Escherichia coli |