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Literature summary for 1.4.3.5 extracted from

  • Tsuge, H.; Ozeki, K.; Sen-Maru, K.; Ohashi, K.
    Purification and properties of pyridoxamine (pyridoxine) 5'-phosphate oxidase from baker's yeast (1979), Agric. Biol. Chem., 43, 1801-1807.
No PubMed abstract available
Search for more literature for 1.4.3.5

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ 2.85 mM, 82% inhibition of pyridoxamine 5'-phosphate oxidation, 27% inhibition of pyridoxine oxidation Saccharomyces cerevisiae
Cu2+ 2.85 mM, 38% inhibition of pyridoxamine 5'-phosphate oxidation, 23% inhibition of pyridoxine oxidation Saccharomyces cerevisiae
Fe2+ 2.85 mM, 44% inhibition of pyridoxamine 5'-phosphate oxidation, 81% inhibition of pyridoxine oxidation Saccharomyces cerevisiae
Fe3+ 2.85 mM, 26% inhibition of pyridoxamine 5'-phosphate oxidation, 50% inhibition of pyridoxine oxidation Saccharomyces cerevisiae
Hg2+ 2.85 mM, 30% inhibition of pyridoxamine 5'-phosphate oxidation, 2% inhibition of pyridoxine oxidation Saccharomyces cerevisiae
Zn2+ 2.85 mM, 92% inhibition of pyridoxamine 5'-phosphate oxidation, 60% inhibition of pyridoxine oxidation Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement for bivalent cations Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.462
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyridoxamine 5'-phosphate + H2O + O2
-
 Saccharomyces cerevisiae pyridoxal 5'-phosphate + NH3 + H2O2
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 above 45°C, loss of activity after 30 min Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 pyridoxine 5'-phosphate Saccharomyces cerevisiae
9
-
 pyridoxamine 5'-phosphate Saccharomyces cerevisiae