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Literature summary for 1.4.3.5 extracted from
- A radiometric assay of pyridoxamine (pyridoxine) 5-phosphate oxidase (1982), Anal. Biochem., 125, 329-334.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | pyridoxamine phosphate oxidase assay applicable for measuring activity in erythrocytes | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00065 | - |
N-Phosphopyridoxyl tryptamine | - |
Rattus norvegicus |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus norvegicus | - |
| erythrocyte | - |
Rattus norvegicus | - |
| heart | - |
Rattus norvegicus | - |
| intestine | - |
Rattus norvegicus | - |
| kidney | - |
Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
| lung | - |
Rattus norvegicus | - |
| muscle | - |
Rattus norvegicus | - |
| spleen | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
development of a radiometric assay method, enzyme activity in erythrocytes and other tissues, overview | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-(5'-phosphopyridoxyl)-tryptamine + FMN + H2O | radio-labeled substrate N-(5'-phosphopyridoxyl)-[3H]tryptamine | Rattus norvegicus | ? | - |
? | |
| N-phosphopyridoxyl tryptamine + H2O + O2 | - |
Rattus norvegicus | pyridoxal 5'-phosphate + tryptamine + H2O2 | - |
? | |
| pyridoxamine 5'-phosphate + H2O + O2 | - |
Rattus norvegicus | pyridoxal 5'-phosphate + NH3 + H2O2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pyridoxamine (pyridoxine) 5'-phosphate oxidase | - |
Rattus norvegicus |
| pyridoxamine (pyridoxine) 5-phosphate:O2 oxidoreductase (deaminating) | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | - |
Rattus norvegicus | |
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Release 2023.1 (January 2023)
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