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Literature summary for 1.4.3.5 extracted from
- Immobilization of yeast pyridoxaminephosphate oxidase to halogenacetyl polysaccharides (1984), Biotechnol. Bioeng., 26, 412-418.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | continuous production of pyridoxal 5'-phosphate with enzyme immobilized to iodo- and bromoacetyl polysaccharides | Saccharomyces cerevisiae |
General Stability
| General Stability | Organism |
|---|---|
| immobilized enzyme is more stable than purified free enzyme against heat and pH change | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyridoxamine 5'-phosphate + H2O + O2 | - |
Saccharomyces cerevisiae | pyridoxal 5'-phosphate + NH3 + H2O2 | - |
? |  |
| pyridoxine 5'-phosphate + H2O + O2 | - |
Saccharomyces cerevisiae | pyridoxal 5'-phosphate + H2O2 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 36 | - |
immobilized enzyme | Saccharomyces cerevisiae |
| 44 | - |
soluble enzyme | Saccharomyces cerevisiae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 50 | immobilized enzyme at 25°C: about 60% of maximal activity, soluble enzyme approx. 40%, immobilized enzyme at 50°C: about 30% of maximal activity, soluble enzyme approx. 20% | Saccharomyces cerevisiae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | - |
pH 7.0, immobilized enzyme stable for 25 days, free enzyme: rapid loss of activity | Saccharomyces cerevisiae |
| 24 | - |
pH 7.0, immobilized enzyme: 50% loss of activity after 10 days, free enzyme: 50% loss of activity after 2-3 days | Saccharomyces cerevisiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.103 | - |
pyridoxamine 5'-phosphate | - |
Saccharomyces cerevisiae | |
| 0.7 | - |
pyridoxine 5'-phosphate | - |
Saccharomyces cerevisiae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
pyridoxine 5'-phosphate, soluble and immobilized enzyme | Saccharomyces cerevisiae |
| 9 | - |
pyridoxamine 5'-phosphate, soluble enzyme | Saccharomyces cerevisiae |
| 9.5 | - |
immobilized enzyme, pyridoxamine 5'-phosphate | Saccharomyces cerevisiae |
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