Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzaldehyde | strong competitive inhibitor | Avena sativa | |
additional information | enzyme activity is not inhibited by pargyline, clorgyline, semicarbazide, or sodium diethyldithiocarbamate | Avena sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
benzylamine | at pH 7.0 and 37°C | Avena sativa | |
0.71 | - |
2-Phenylethylamine | at pH 7.0 and 37°C | Avena sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
1 * 78000 + 1 * 58000 + 1 * 55000 + 1 * 32000, SDS-PAGE | Avena sativa |
55000 | - |
1 * 78000 + 1 * 58000 + 1 * 55000 + 1 * 32000, SDS-PAGE | Avena sativa |
58000 | - |
1 * 78000 + 1 * 58000 + 1 * 55000 + 1 * 32000, SDS-PAGE | Avena sativa |
78000 | - |
1 * 78000 + 1 * 58000 + 1 * 55000 + 1 * 32000, SDS-PAGE | Avena sativa |
220000 | - |
gel filtration | Avena sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Avena sativa | - |
cultivar Miechikara | - |
Purification (Comment) | Organism |
---|---|
Sephadex G-25 gel filtration, DEAE Sephacel gel filtration, hydroxyapatitecolumn chromatography, Mono Q column chromatography, and TSK-GEL filtration | Avena sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Avena sativa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00015 | - |
crude extract, at pH 7.0 and 37°C | Avena sativa |
0.02 | - |
125fold purified enzyme, at pH 7.0 and 37°C | Avena sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phenylethylamine + H2O + O2 | the enzyme shows high substrate specificity for phenethylamine (100% activity) | Avena sativa | 2-phenylacetaldehyde + NH3 + H2O2 | - |
? | |
benzylamine + H2O + O2 | 60% activity compared to 2-phenylethylamine | Avena sativa | benzaldehyde + NH3 + H2O2 | - |
? | |
additional information | no activity with tyramine, tryptamine, serotonin, dopamine, ethanolamine, histamine, ethylamine, n-propylamine, n-butylamine, n-pentylamine, putrescine, and spermidine | Avena sativa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | 1 * 78000 + 1 * 58000 + 1 * 55000 + 1 * 32000, SDS-PAGE | Avena sativa |
Synonyms | Comment | Organism |
---|---|---|
MAO | - |
Avena sativa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Avena sativa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 80 | the purified enzyme retains its activity at temperatures up to 50°C, but loses activity quickly after 10 min incubation at temperaturesabove 50°C | Avena sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Avena sativa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the enzyme contains 2 mol of FAD per 220000 g of enzyme | Avena sativa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
benzaldehyde | at pH 7.0 and 37°C | Avena sativa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Avena sativa | isoelectric focusing | - |
6.3 |
Organism | Comment | Expression |
---|---|---|
Avena sativa | enzyme activity increases up to day 3 after germination, thereafter decreasing again | up |