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Literature summary for 1.4.3.4 extracted from

  • Dittmann, K.; Riese, U.; Hamburger, M.
    HPLC-based bioactivity profiling of plant extracts: a kinetic assay for the identification of monoamine oxidase-A inhibitors using human recombinant monoamine oxidase-A (2004), Phytochemistry, 65, 2885-2891.
    View publication on PubMed

Application

Application Comment Organism
analysis kinetic assay for detection of MAO-A inhibitors in plant extracts using recombinant MAO-A expressed as GST-fusion protein in yesat Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
monoamine oxidase A is cloned into the high copy yeast expression vector pEG-KT, to generate a GST MAO-A fusion protein Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Clorgyline IC50: 0.000014 mM Homo sapiens
Deprenyl IC50: 0.020 mM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
kynuramine
-
Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
x * 65000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-
placenta
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
kynuramine + H2O + O2
-
Homo sapiens 3-(2-aminophenyl)-3-oxopropanal + NH3 + H2O2
-
?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
MAO-A
-
Homo sapiens
monoamine oxidase A
-
Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000014
-
IC50: 0.000014 mM Homo sapiens Clorgyline
0.02
-
IC50: 0.020 mM Homo sapiens Deprenyl