Cloned (Comment) | Organism |
---|---|
functional enzyme overexpression in Escherichia coli strain BL21(DE3), method optimization and upscaling, detailed overview | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | - |
Homo sapiens | 5777 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-alanine + H2O + O2 | Homo sapiens | - |
pyruvate + NH3 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
flavoprotein | - |
Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3) | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8 | - |
purified recombinant enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-alanine + H2O + O2 | - |
Homo sapiens | pyruvate + NH3 + H2O2 | - |
? | |
additional information | hDAAO exhibits optimal activity toward neutral D-amino acids and marginal activity toward basic ones, while acidic D-amino acids are not oxidized, structure-function relationship analysis, overview | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | structure-function relationship analysis, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
DAAO | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | DAAO is a flavoprotein, the cofactor is loosely bound | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | DAAO plays a key role in the pathophysiology of schizophrenia | Homo sapiens |