Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Rhodotorula toruloides |
Protein Variants | Comment | Organism |
---|---|---|
M213G | mutant shows lower activity and substrate specificity constant on the natural aromatic D-amino acids and higher acitivy on artificial amino acids compared to the wild type enzyme | Rhodotorula toruloides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
D-2-naphthyl-glycine | wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C | Rhodotorula toruloides | |
0.03 | - |
D-1-naphthyl-alanine | mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C | Rhodotorula toruloides | |
0.03 | - |
D-2-naphthyl-glycine | mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C | Rhodotorula toruloides | |
0.03 | - |
D-4-chloro-phenylglycine | wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C | Rhodotorula toruloides | |
0.04 | - |
D-2-naphthyl-alanine | wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C | Rhodotorula toruloides | |
0.04 | - |
D-1-naphthyl-alanine | wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C | Rhodotorula toruloides | |
0.05 | - |
D-1-naphthyl-glycine | mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C | Rhodotorula toruloides | |
0.06 | - |
D-2-naphthyl-alanine | mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C | Rhodotorula toruloides | |
0.13 | - |
D-2-chloro-phenylglycine | wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C | Rhodotorula toruloides | |
0.15 | - |
D-homo-phenylalanine | mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C | Rhodotorula toruloides | |
0.2 | - |
D-phenylalanine | mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C | Rhodotorula toruloides | |
0.2 | - |
D-4-chloro-phenylglycine | mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C | Rhodotorula toruloides | |
0.3 | - |
D-phenylalanine | wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C | Rhodotorula toruloides | |
0.3 | - |
D-homo-phenylalanine | wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C | Rhodotorula toruloides | |
0.33 | - |
D-1-naphthyl-glycine | wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C | Rhodotorula toruloides | |
0.4 | - |
D-2-chloro-phenylglycine | mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C | Rhodotorula toruloides | |
0.5 | - |
D-2-fluoro-phenylglycine | mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C | Rhodotorula toruloides | |
0.5 | - |
D-2-fluoro-phenylglycine | wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C | Rhodotorula toruloides | |
0.6 | - |
D-4-fluoro-phenylglycine | mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C | Rhodotorula toruloides | |
0.7 | - |
D-4-fluoro-phenylglycine | wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C | Rhodotorula toruloides | |
1.6 | - |
D-4-hydroxy-phenylglycine | mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C | Rhodotorula toruloides | |
2.1 | - |
D-4-hydroxy-phenylglycine | wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C | Rhodotorula toruloides | |
3.5 | - |
D-phenylglycine | wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C | Rhodotorula toruloides | |
5.2 | - |
D-phenylglycine | mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C | Rhodotorula toruloides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-alanine + H2O + O2 | Rhodotorula toruloides | - |
pyruvate + NH3 + H2O2 | - |
? | |
D-phenylalanine + H2O + O2 | Rhodotorula toruloides | - |
phenylpyruvate + NH3 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodotorula toruloides | P80324 | - |
- |
Purification (Comment) | Organism |
---|---|
Hitrap chelating chromatography | Rhodotorula toruloides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-1-naphthyl-alanine + H2O + O2 | - |
Rhodotorula toruloides | ? | - |
? | |
D-1-naphthyl-glycine + H2O + O2 | - |
Rhodotorula toruloides | ? | - |
? | |
D-2-chloro-phenylglycine + H2O + O2 | - |
Rhodotorula toruloides | (2-chlorophenyl)acetic acid + NH3 + H2O2 | - |
? | |
D-2-fluoro-phenylglycine + H2O + O2 | - |
Rhodotorula toruloides | (2-fluorophenyl)acetic acid + NH3 + H2O2 | - |
? | |
D-2-naphthyl-alanine + H2O + O2 | - |
Rhodotorula toruloides | ? | - |
? | |
D-2-naphthyl-glycine + H2O + O2 | - |
Rhodotorula toruloides | ? | - |
? | |
D-4-chloro-phenylglycine + H2O + O2 | - |
Rhodotorula toruloides | (4-chlorophenyl)acetic acid + NH3 + H2O2 | - |
? | |
D-4-fluoro-phenylglycine + H2O + O2 | - |
Rhodotorula toruloides | (4-fluorophenyl)acetic acid + NH3 + H2O2 | - |
? | |
D-4-hydroxy-phenylglycine + H2O + O2 | - |
Rhodotorula toruloides | (4-hydroxyphenyl)acetic acid + NH3 + H2O2 | - |
? | |
D-alanine + H2O + O2 | - |
Rhodotorula toruloides | pyruvate + NH3 + H2O2 | - |
? | |
D-homo-phenylalanine + H2O + O2 | - |
Rhodotorula toruloides | ? | - |
? | |
D-phenylalanine + H2O + O2 | - |
Rhodotorula toruloides | phenylpyruvate + NH3 + H2O2 | - |
? | |
D-phenylalanine + H2O + O2 | - |
Rhodotorula toruloides | ? | - |
? | |
D-phenylglycine + H2O + O2 | - |
Rhodotorula toruloides | benzoylformic acid + NH3 + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
D-amino acid oxidase | - |
Rhodotorula toruloides |
DAAO | - |
Rhodotorula toruloides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | FAD-dependent enzyme | Rhodotorula toruloides |