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Literature summary for 1.4.3.3 extracted from

  • Caligiuri, A.; DArrigo, P.; Rosini, E.; Pedrocchi-Fantoni, G.; Tessaro, D.; Molla, G.; Servi, S.; Pollegioni, L.
    Activity of yeast D-amino acid oxidase on aromatic unnatural amino acids (2008), J. Mol. Catal. B, 50, 93-98.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Rhodotorula toruloides

Protein Variants

Protein Variants Comment Organism
M213G mutant shows lower activity and substrate specificity constant on the natural aromatic D-amino acids and higher acitivy on artificial amino acids compared to the wild type enzyme Rhodotorula toruloides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
D-2-naphthyl-glycine wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
0.03
-
D-1-naphthyl-alanine mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
0.03
-
D-2-naphthyl-glycine mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
0.03
-
D-4-chloro-phenylglycine wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
0.04
-
D-2-naphthyl-alanine wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
0.04
-
D-1-naphthyl-alanine wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
0.05
-
D-1-naphthyl-glycine mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
0.06
-
D-2-naphthyl-alanine mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
0.13
-
D-2-chloro-phenylglycine wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
0.15
-
D-homo-phenylalanine mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
0.2
-
D-phenylalanine mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
0.2
-
D-4-chloro-phenylglycine mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
0.3
-
D-phenylalanine wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
0.3
-
D-homo-phenylalanine wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
0.33
-
D-1-naphthyl-glycine wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
0.4
-
D-2-chloro-phenylglycine mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
0.5
-
D-2-fluoro-phenylglycine mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
0.5
-
D-2-fluoro-phenylglycine wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
0.6
-
D-4-fluoro-phenylglycine mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
0.7
-
D-4-fluoro-phenylglycine wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
1.6
-
D-4-hydroxy-phenylglycine mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
2.1
-
D-4-hydroxy-phenylglycine wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
3.5
-
D-phenylglycine wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides
5.2
-
D-phenylglycine mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C Rhodotorula toruloides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-alanine + H2O + O2 Rhodotorula toruloides
-
pyruvate + NH3 + H2O2
-
?
D-phenylalanine + H2O + O2 Rhodotorula toruloides
-
phenylpyruvate + NH3 + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Rhodotorula toruloides P80324
-
-

Purification (Commentary)

Purification (Comment) Organism
Hitrap chelating chromatography Rhodotorula toruloides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-1-naphthyl-alanine + H2O + O2
-
Rhodotorula toruloides ?
-
?
D-1-naphthyl-glycine + H2O + O2
-
Rhodotorula toruloides ?
-
?
D-2-chloro-phenylglycine + H2O + O2
-
Rhodotorula toruloides (2-chlorophenyl)acetic acid + NH3 + H2O2
-
?
D-2-fluoro-phenylglycine + H2O + O2
-
Rhodotorula toruloides (2-fluorophenyl)acetic acid + NH3 + H2O2
-
?
D-2-naphthyl-alanine + H2O + O2
-
Rhodotorula toruloides ?
-
?
D-2-naphthyl-glycine + H2O + O2
-
Rhodotorula toruloides ?
-
?
D-4-chloro-phenylglycine + H2O + O2
-
Rhodotorula toruloides (4-chlorophenyl)acetic acid + NH3 + H2O2
-
?
D-4-fluoro-phenylglycine + H2O + O2
-
Rhodotorula toruloides (4-fluorophenyl)acetic acid + NH3 + H2O2
-
?
D-4-hydroxy-phenylglycine + H2O + O2
-
Rhodotorula toruloides (4-hydroxyphenyl)acetic acid + NH3 + H2O2
-
?
D-alanine + H2O + O2
-
Rhodotorula toruloides pyruvate + NH3 + H2O2
-
?
D-homo-phenylalanine + H2O + O2
-
Rhodotorula toruloides ?
-
?
D-phenylalanine + H2O + O2
-
Rhodotorula toruloides phenylpyruvate + NH3 + H2O2
-
?
D-phenylalanine + H2O + O2
-
Rhodotorula toruloides ?
-
?
D-phenylglycine + H2O + O2
-
Rhodotorula toruloides benzoylformic acid + NH3 + H2O2
-
?

Synonyms

Synonyms Comment Organism
D-amino acid oxidase
-
Rhodotorula toruloides
DAAO
-
Rhodotorula toruloides

Cofactor

Cofactor Comment Organism Structure
FAD FAD-dependent enzyme Rhodotorula toruloides