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Literature summary for 1.4.3.3 extracted from

  • Rosini, E.; Molla, G.; Rossetti, C.; Pilone, M.S.; Pollegioni, L.; Sacchi, S.
    A biosensor for all D-amino acids using evolved D-amino acid oxidase (2008), J. Biotechnol., 135, 377-384.
    View publication on PubMed
Search for more literature for 1.4.3.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Rhodotorula toruloides

Protein Variants

Protein Variants Comment Organism
M213E increased overall activity compared to the wild type enzyme Rhodotorula toruloides
M213G increased overall activity compared to the wild type enzyme Rhodotorula toruloides
M213R increased overall activity compared to the wild type enzyme Rhodotorula toruloides
Q144R increased overall activity compared to the wild type enzyme Rhodotorula toruloides
Q339E decreased overall activity compared to the wild type enzyme Rhodotorula toruloides
T60A/Q144R/K152E increased overall activity compared to the wild type enzyme Rhodotorula toruloides
Y238R increased overall activity compared to the wild type enzyme Rhodotorula toruloides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 D-methionine mutant enzyme T60A/Q144R/K152E, apparent value, at 25°C and pH 8.5 Rhodotorula toruloides
0.6
-
 D-alanine mutant enzyme T60A/Q144R/K152E, apparent value, at 25°C and pH 8.5 Rhodotorula toruloides
0.6
-
 D-methionine wild type enzyme, apparent value, at 25°C and pH 8.5 Rhodotorula toruloides
0.8
-
 D-methionine mutant enzyme M213G, apparent value, at 25°C and pH 8.5 Rhodotorula toruloides
0.9
-
 D-alanine wild type enzyme, apparent value, at 25°C and pH 8.5 Rhodotorula toruloides
1.2
-
 D-methionine mutant enzyme M213R, apparent value, at 25°C and pH 8.5 Rhodotorula toruloides
1.3
-
 D-valine immobilized wild type enzyme, at 25°C Rhodotorula toruloides
2.5
-
 D-glutamine mutant enzyme M213G, apparent value, at 25°C and pH 8.5 Rhodotorula toruloides
4.9
-
 D-alanine mutant enzyme M213G, apparent value, at 25°C and pH 8.5 Rhodotorula toruloides
5.1
-
 D-glutamate mutant enzyme M213G, apparent value, at 25°C and pH 8.5 Rhodotorula toruloides
5.4
-
 D-glutamine mutant enzyme M213R, apparent value, at 25°C and pH 8.5 Rhodotorula toruloides
5.4
-
 D-glutamine wild type enzyme, apparent value, at 25°C and pH 8.5 Rhodotorula toruloides
5.6
-
 D-proline immobilized wild type enzyme, at 25°C Rhodotorula toruloides
6
-
 D-glutamine mutant enzyme T60A/Q144R/K152E, apparent value, at 25°C and pH 8.5 Rhodotorula toruloides
8
-
 D-Lysine mutant enzyme M213G, apparent value, at 25°C and pH 8.5 Rhodotorula toruloides
12.5
-
 D-proline free wild type enzyme, at 25°C Rhodotorula toruloides
14.6
-
 D-Lysine wild type enzyme, apparent value, at 25°C and pH 8.5 Rhodotorula toruloides
16.6
-
 D-Lysine mutant enzyme T60A/Q144R/K152E, apparent value, at 25°C and pH 8.5 Rhodotorula toruloides
18.5
-
 D-alanine mutant enzyme M213R, apparent value, at 25°C and pH 8.5 Rhodotorula toruloides
18.9
-
 D-valine free wild type enzyme, at 25°C Rhodotorula toruloides
21.1
-
 D-glutamate mutant enzyme M213R, apparent value, at 25°C and pH 8.5 Rhodotorula toruloides
76
-
 D-glutamate wild type enzyme, apparent value, at 25°C and pH 8.5 Rhodotorula toruloides
101
-
 D-glutamate mutant enzyme T60A/Q144R/K152E, apparent value, at 25°C and pH 8.5 Rhodotorula toruloides

Organism

Organism UniProt Comment Textmining
Rhodotorula toruloides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Hitrap chelating chromatography Rhodotorula toruloides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cephalosporin C + H2O + O2
-
 Rhodotorula toruloides 7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
-
 ?
D-alanine + H2O + O2
-
 Rhodotorula toruloides pyruvate + NH3 + H2O2
-
 ?
D-arginine + H2O + O2
-
 Rhodotorula toruloides 5-guanidino-2-oxopentanoic acid + NH3 + H2O2
-
 ?
D-asparagine + H2O + O2
-
 Rhodotorula toruloides 2-oxosuccinamic acid + NH3 + H2O2
-
 ?
D-aspartate + H2O + O2
-
 Rhodotorula toruloides oxaloacetate + NH3 + H2O2
-
 ?
D-cysteine + H2O + O2
-
 Rhodotorula toruloides 2-oxo-3-thiopropionic acid + NH3 + H2O2
-
 ?
D-glutamate + H2O + O2
-
 Rhodotorula toruloides alpha-ketoglutarate + NH3 + H2O2
-
 ?
D-glutamine + H2O + O2
-
 Rhodotorula toruloides 2-oxoglutaramate + NH3 + H2O2
-
 ?
D-histidine + H2O + O2
-
 Rhodotorula toruloides 3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + H2O2
-
 ?
D-isoleucine + H2O + O2
-
 Rhodotorula toruloides 3-methyl-2-oxopentanoate + NH3 + H2O2
-
 ?
D-leucine + H2O + O2
-
 Rhodotorula toruloides 4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
 ?
D-lysine + H2O + O2
-
 Rhodotorula toruloides 6-amino-2-oxohexanoic acid + NH3 + H2O2
-
 ?
D-methionine + H2O + O2
-
 Rhodotorula toruloides 4-methylthio-2-oxobutanoic acid + NH3 + H2O2
-
 ?
D-phenylalanine + H2O + O2
-
 Rhodotorula toruloides phenylpyruvate + NH3 + H2O2
-
 ?
D-proline + H2O + O2
-
 Rhodotorula toruloides 2-oxopentanoic acid + NH3 + H2O2
-
 ?
D-serine + H2O + O2
-
 Rhodotorula toruloides 2-oxo-3-hydroxypropionic acid + NH3 + H2O2
-
 ?
D-threonine + H2O + O2
-
 Rhodotorula toruloides 2-oxo-3-hydroxybutyric acid + NH3 + H2O2
-
 ?
D-tryptophan + H2O + O2
-
 Rhodotorula toruloides indol-3-pyruvate + NH3 + H2O2
-
 ?
D-tyrosine + H2O + O2
-
 Rhodotorula toruloides 4-hydroxyphenylpyruvic acid + NH3 + H2O2
-
 ?
D-valine + H2O + O2
-
 Rhodotorula toruloides alpha-ketoisovaleric acid + NH3 + H2O2
-
 ?
glycine + 2 H2O + O2
-
 Rhodotorula toruloides 2 formic acid + NH3 + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
D-amino acid oxidase
-
 Rhodotorula toruloides
DAAO
-
 Rhodotorula toruloides

Cofactor

Cofactor Comment Organism Structure
FAD FAD-containing flavoenzyme Rhodotorula toruloides