Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.4.3.3 extracted from

  • Fang, J.; Deng, D.; Nakamura, H.; Akuta, T.; Qin, H.; Iyer, A.K.; Greish, K.; Maeda, H.
    Oxystress inducing antitumor therapeutics via tumor-targeted delivery of PEG-conjugated D-amino acid oxidase (2008), Int. J. Cancer, 122, 1135-1144.
    View publication on PubMed

Application

Application Comment Organism
medicine polyethylene glycol-conjugated D-amino acid oxidase exhibits potent antitumor activity by generating toxic reactive oxygen species, namely oxidation therapy, subsequently shows remarkable antitumor effect on murine sarcoma 180 solid tumor Sus scrofa

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21 (DE3) Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
2 * 39000, native D-amino acid oxidase, SDS-PAGE Sus scrofa
65000
-
2 * 65000, recombinant polyethylene glycol-conjugated D-amino acid oxidase, SDS-PAGE Sus scrofa
113000
-
recombinant polyethylene glycol-conjugated D-amino acid oxidase, gel filtration Sus scrofa

Organic Solvent Stability

Organic Solvent Comment Organism
Triton X-114 less than 10% decrease of enzyme activity after Triton X-114 treatment, sonication, lyophilization or repeated freezing-thawing Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap DEAE column chromatography Sus scrofa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.6
-
recombinant polyethylene glycol-conjugated D-amino acid oxidase, at 25°C in 0.1 M Tris-HCl buffer (pH 8.2) Sus scrofa
5.3
-
native enzyme, at 25°C in 0.1 M Tris-HCl buffer (pH 8.2) Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cephalosporin C + H2O + O2
-
Sus scrofa 7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
-
?
D-alanine + H2O + O2
-
Sus scrofa pyruvate + NH3 + H2O2
-
?
D-methionine + H2O + O2
-
Sus scrofa 4-methylthio-2-oxobutanoic acid + NH3 + H2O2
-
?
D-proline + H2O + O2
-
Sus scrofa 2-oxopentanoic acid + NH3 + H2O2
-
?
glycine + 2 H2O + O2
-
Sus scrofa 2 formic acid + NH3 + H2O2
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 39000, native D-amino acid oxidase, SDS-PAGE Sus scrofa
homodimer 2 * 65000, recombinant polyethylene glycol-conjugated D-amino acid oxidase, SDS-PAGE Sus scrofa

Synonyms

Synonyms Comment Organism
D-amino acid oxidase
-
Sus scrofa
PEG-DAO
-
Sus scrofa

Cofactor

Cofactor Comment Organism Structure
FAD
-
Sus scrofa