Literature summary for 1.4.3.3 extracted from

Setoyama, C.; Nishina, Y.; Mizutani, H.; Miyahara, I.; Hirotsu, K.; Kamiya, N.; Shiga, K.; Miura, R.
Engineering the substrate specificity of porcine kidney D-amino acid oxidase by mutagenesis of the active-site lid (2006), J. Biochem., 139, 873-879.

Protein Variants

Protein Variants	Comment	Organism
E220D/Y224G	kcat/KM for D-Arg is 5fold higher than wild-type value, kcat/Km for L-Lys is 2.91fold higher than wild-type value, kcat/KM for D-Ala is 124fold lower than wild-type value, kcat/Km for D-Ser is more than 110fold lower than wild-type value, kcat/Km for D-Pro is 17.5fold lower than wild-type value, kcat/Km for D-Val is 12fold lower than wild-type value, kcat/KM for D-Met is 1.5fold higher than wild-type value, kcat/KM for D-Phe is 3.3fold higher than wild-type value	Sus scrofa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.56	-	D-Pro	wild-type enzyme	Sus scrofa
0.63	-	D-Val	wild-type enzyme	Sus scrofa
0.65	-	D-Met	wild-type enzyme	Sus scrofa
0.67	-	D-Met	mutant enzyme E220D/Y224G	Sus scrofa
0.67	-	D-Met	mutant enzyme R221D/Y224G	Sus scrofa
0.77	-	D-Ala	wild-type enzyme	Sus scrofa
0.77	-	D-Phe	mutant enzyme R221D/Y224G	Sus scrofa
1.4	-	D-Phe	wild-type enzyme	Sus scrofa
1.4	-	D-Phe	mutant enzyme E220D/Y224G	Sus scrofa
3	-	D-Arg	mutant enzyme E220D/Y224G	Sus scrofa
3	-	D-Arg	mutant enzyme R221D/Y224G	Sus scrofa
3.1	-	D-Val	mutant enzyme E220D/Y224G	Sus scrofa
3.3	-	D-Ser	wild-type enzyme	Sus scrofa
3.7	-	D-Val	mutant enzyme R221D/Y224G	Sus scrofa
4.1	-	D-Pro	mutant enzyme E220D/Y224G	Sus scrofa
7.7	-	D-Pro	mutant enzyme R221D/Y224G	Sus scrofa
10	-	D-Lys	wild-type enzyme	Sus scrofa
13	-	D-Arg	wild-type enzyme	Sus scrofa
14	-	D-Lys	mutant enzyme E220D/Y224G	Sus scrofa
20	-	D-Lys	mutant enzyme R221D/Y224G	Sus scrofa
44	-	D-Ala	mutant enzyme E220D/Y224G	Sus scrofa
46	-	D-Ala	mutant enzyme R221D/Y224G	Sus scrofa

Organism

Organism	UniProt	Comment	Textmining
Sus scrofa	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
kidney	-	Sus scrofa	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
D-Ala + H2O + O2	-	Sus scrofa	pyruvate + NH3 + H2O2	-	?
D-Arg + H2O + O2	-	Sus scrofa	5-guanidino-2-oxopentanoic acid + NH3 + H2O2	-	?
D-Lys + H2O + O2	-	Sus scrofa	6-amino-2-oxohexanoic acid + NH3 + H2O2	-	?
D-Met + H2O + O2	-	Sus scrofa	4-methylthio-2-oxobutanoate + NH3 + H2O2	-	?
D-Phe + H2O + O2	-	Sus scrofa	phenylpyruvate + NH3 + H2O2	-	?
D-Pro + H2O + O2	-	Sus scrofa	2-oxopentanoate + NH3 + H2O2	-	?
D-Ser + H2O + O2	-	Sus scrofa	2-oxo-3-hydroxypropionate + NH3 + H2O2	-	?
D-Val + H2O + O2	-	Sus scrofa	alpha-ketoisovalerate + NH3 + H2O2	-	?
additional information	activity with D-Asp is not detectable	Sus scrofa	?	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.052	2.1	D-Pro	wild-type enzyme	Sus scrofa
0.25	-	D-Val	mutant enzyme G222D/Y224G	Sus scrofa
0.4	-	D-Phe	mutant enzyme E220D/Y224G	Sus scrofa
0.433	-	D-Val	mutant enzymeY224G	Sus scrofa
0.75	-	D-Ala	mutant enzyme R221D/Y224G	Sus scrofa
0.75	-	D-Val	mutant enzyme R221D/Y224G	Sus scrofa
0.8	-	D-Lys	wild-type enzyme	Sus scrofa
1.05	-	D-Val	mutant enzyme E220D/Y224G	Sus scrofa
1.33	-	D-Met	mutant enzyme I223D/Y224G	Sus scrofa
1.38	-	D-Met	mutant enzymeY224G	Sus scrofa
1.67	-	D-Pro	mutant enzymeY224G	Sus scrofa
1.83	-	D-Arg	mutant enzyme I223D/Y224G	Sus scrofa
2	-	D-Arg	mutant enzymeY224G	Sus scrofa
2.2	-	D-Ser	mutant enzyme E220D/Y224G	Sus scrofa
2.33	-	D-Lys	mutant enzymeY224G	Sus scrofa
2.5	-	D-Val	wild-type enzyme	Sus scrofa
2.5	-	D-Met	mutant enzyme G222D/Y224G	Sus scrofa
3	-	D-Ser	wild-type enzyme	Sus scrofa
3	-	D-Ala	mutant enzyme E220D/Y224G	Sus scrofa
3	-	D-Phe	mutant enzyme I223D/Y224G	Sus scrofa
3	-	D-Lys	mutant enzyme I223D/Y224G	Sus scrofa
3.17	-	D-Phe	mutant enzymeY224G	Sus scrofa
3.33	-	D-Lys	mutant enzyme E220D/Y224G	Sus scrofa
3.5	-	D-Arg	wild-type enzyme	Sus scrofa
3.5	-	D-Phe	mutant enzyme G222D/Y224G	Sus scrofa
3.5	-	D-Pro	mutant enzyme G222D/Y224G	Sus scrofa
3.83	-	D-Pro	mutant enzyme R221D/Y224G	Sus scrofa
4	-	D-Arg	mutant enzyme E220D/Y224G	Sus scrofa
4	-	D-Arg	mutant enzyme G222D/Y224G	Sus scrofa
4.33	-	D-Pro	mutant enzyme E220D/Y224G	Sus scrofa
5	-	D-Lys	mutant enzyme G222D/Y224G	Sus scrofa
5	-	D-Lys	mutant enzyme R221D/Y224G	Sus scrofa
6.5	-	D-Ala	wild-type enzyme	Sus scrofa
6.83	-	D-Met	wild-type enzyme	Sus scrofa
7.5	-	D-Arg	mutant enzyme R221D/Y224G	Sus scrofa
9	-	D-Met	mutant enzyme R221D/Y224G	Sus scrofa
10.33	-	D-Pro	wild-type enzyme	Sus scrofa
10.5	-	D-Met	mutant enzyme E220D/Y224G	Sus scrofa
13	-	D-Phe	mutant enzyme R221D/Y224G	Sus scrofa
16.7	-	D-Phe	wild-type enzyme	Sus scrofa
53.33	-	D-Phe	mutant enzyme E220D/Y224G	Sus scrofa