Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Rhodotorula toruloides |
Protein Variants | Comment | Organism |
---|---|---|
R285A | decreased activity with D-amino acids | Rhodotorula toruloides |
R285D | decreased activity with D-amino acids | Rhodotorula toruloides |
R285K | decreased activity with D-amino acids | Rhodotorula toruloides |
R285Q | decreased activity with D-amino acids | Rhodotorula toruloides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
O2 | mutant R285K | Rhodotorula toruloides | |
0.08 | - |
O2 | mutant R285A | Rhodotorula toruloides | |
2.3 | - |
O2 | wild-type | Rhodotorula toruloides | |
2.6 | - |
D-alanine | wild-type | Rhodotorula toruloides | |
310 | - |
D-alanine | mutant R285A | Rhodotorula toruloides | |
800 | - |
D-alanine | mutant R285K | Rhodotorula toruloides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodotorula toruloides | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Storage Stability | Organism |
---|---|
-20°C, several months | Rhodotorula toruloides |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
D-alanine | mutant R285A | Rhodotorula toruloides | |
0.8 | - |
D-alanine | mutant R285K | Rhodotorula toruloides | |
350 | - |
D-alanine | wild-type | Rhodotorula toruloides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Rhodotorula toruloides |