Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.4.3.3 extracted from

  • Simonetta, M.P.; Vanoni, M.A.; Casalin, P.
    Purification and properties of D-amino-acid oxidase, an inducible flavoenzyme from Rhodotorula gracilis (1987), Biochim. Biophys. Acta, 914, 136-142.
No PubMed abstract available
Search for more literature for 1.4.3.3

General Stability

General Stability Organism
cetylpyridinium bromide stabilizes Rhodotorula toruloides
glycerol, 10%, stabilizes Rhodotorula toruloides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 2 * 37000, SDS-PAGE Rhodotorula toruloides
79000
-
 gel filtration Rhodotorula toruloides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-alanine + H2O + O2 Rhodotorula toruloides
-
 pyruvic acid + NH3 + H2O2
-
 ?
D-asparagine + H2O + O2 Rhodotorula toruloides
-
 2-oxosuccinamic acid + NH3 + H2O2
-
 ?
D-cysteine + H2O + O2 Rhodotorula toruloides
-
 2-oxo-3-thiopropionic acid + NH3 + H2O2
-
 ?
D-glutamine + H2O + O2 Rhodotorula toruloides
-
 2-oxoglutaramate + NH3 + H2O2
-
 ?
D-isoleucine + H2O + O2 Rhodotorula toruloides
-
 3-methyl-2-oxopentanoic acid + NH3 + H2O2
-
 ?
D-leucine + H2O + O2 Rhodotorula toruloides
-
 4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
 ?
D-methionine + H2O + O2 Rhodotorula toruloides
-
 4-methylthio-2-oxobutanoic acid + NH3 + H2O2
-
 ?
D-phenylalanine + H2O + O2 Rhodotorula toruloides
-
 phenylpyruvic acid + NH3 + H2O2
-
 ?
D-proline + H2O + O2 Rhodotorula toruloides
-
 2-oxopentanoic acid + NH3 + H2O2
-
 ?
D-serine + H2O + O2 Rhodotorula toruloides
-
 2-oxo-3-hydroxypropionic acid + NH3 + H2O2
-
 ?
D-threonine + H2O + O2 Rhodotorula toruloides
-
 2-oxo-3-hydroxybutyric acid + NH3 + H2O2
-
 ?
D-tryptophan + H2O + O2 Rhodotorula toruloides
-
 indol-3-pyruvic acid + NH3 + H2O2
-
 ?
D-valine + H2O + O2 Rhodotorula toruloides
-
 alpha-ketoisovaleric acid + NH3 + H2O2
-
 ?
phenylglycine + H2O + O2 Rhodotorula toruloides
-
 benzoylformic acid + NH3 + H2O2
-
 ?
thiazolidine-2-carboxylic acid + H2O + O2 Rhodotorula toruloides
-
 ? + NH3 + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodotorula toruloides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rhodotorula toruloides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
57.8
-
-
 Rhodotorula toruloides

Storage Stability

Storage Stability Organism
-80°C, 2-3 months, stable Rhodotorula toruloides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-alanine + H2O + O2
-
 Rhodotorula toruloides pyruvic acid + NH3 + H2O2
-
 ?
D-asparagine + H2O + O2
-
 Rhodotorula toruloides 2-oxosuccinamic acid + NH3 + H2O2
-
 ?
D-cysteine + H2O + O2
-
 Rhodotorula toruloides 2-oxo-3-thiopropionic acid + NH3 + H2O2
-
 ?
D-glutamine + H2O + O2
-
 Rhodotorula toruloides 2-oxoglutaramate + NH3 + H2O2
-
 ?
D-isoleucine + H2O + O2
-
 Rhodotorula toruloides 3-methyl-2-oxopentanoic acid + NH3 + H2O2
-
 ?
D-leucine + H2O + O2
-
 Rhodotorula toruloides 4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
 ?
D-methionine + H2O + O2
-
 Rhodotorula toruloides 4-methylthio-2-oxobutanoic acid + NH3 + H2O2
-
 ?
D-phenylalanine + H2O + O2
-
 Rhodotorula toruloides phenylpyruvic acid + NH3 + H2O2
-
 ?
D-proline + H2O + O2
-
 Rhodotorula toruloides 2-oxopentanoic acid + NH3 + H2O2
-
 ?
D-serine + H2O + O2
-
 Rhodotorula toruloides 2-oxo-3-hydroxypropionic acid + NH3 + H2O2
-
 ?
D-threonine + H2O + O2
-
 Rhodotorula toruloides 2-oxo-3-hydroxybutyric acid + NH3 + H2O2
-
 ?
D-tryptophan + H2O + O2
-
 Rhodotorula toruloides indol-3-pyruvic acid + NH3 + H2O2
-
 ?
D-valine + H2O + O2
-
 Rhodotorula toruloides alpha-ketoisovaleric acid + NH3 + H2O2
-
 ?
phenylglycine + H2O + O2
-
 Rhodotorula toruloides benzoylformic acid + NH3 + H2O2
-
 ?
thiazolidine-2-carboxylic acid + H2O + O2
-
 Rhodotorula toruloides ? + NH3 + H2O2
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 37000, SDS-PAGE Rhodotorula toruloides

pH Stability

pH Stability pH Stability Maximum Comment Organism
8
-
 unstable above Rhodotorula toruloides

Cofactor

Cofactor Comment Organism Structure
FAD tightly bound Rhodotorula toruloides
FAD flavoprotein Rhodotorula toruloides