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Literature summary for 1.4.3.21 extracted from

  • Sun, J.; Morita, H.; Chen, G.; Noguchi, H.; Abe, I.
    Molecular cloning and characterization of copper amine oxidase from Huperzia serrata (2012), Bioorg. Med. Chem. Lett., 22, 5784-5790.
    View publication on PubMed
Search for more literature for 1.4.3.21

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Huperzia serrata

Protein Variants

Protein Variants Comment Organism
F190Y the mutant loses the activity within the range of pH 6.8-8.5 Huperzia serrata
F384G the mutant loses the activity within the range of pH 6.8-8.5 Huperzia serrata
I160F the mutant loses the activity within the range of pH 6.8-8.5 Huperzia serrata
S406T the mutant loses the activity within the range of pH 6.8-8.5 Huperzia serrata
Y320F the mutant loses the activity within the range of pH 6.8-8.5 Huperzia serrata
Y326F the mutant loses the activity within the range of pH 6.8-8.5 Huperzia serrata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 cadaverine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
0.5
-
 benzylamine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
0.7
-
 histamine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
0.8
-
 spermidine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
1.2
-
 putrescine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
1.2
-
 3-aminomethylpyridine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
1.7
-
 2-aminoethylpyridine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
1.9
-
 4-aminomethylpyridine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ required for activity Huperzia serrata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
77676
-
 2 * 77676, calculated from amino acid sequence Huperzia serrata
78000
-
 2 * 78000, SDS-PAGE Huperzia serrata
162000
-
 gel filtration Huperzia serrata

Organism

Organism UniProt Comment Textmining
Huperzia serrata I6NC69 Chinese club moss
-

Purification (Commentary)

Purification (Comment) Organism
Ni-chelating affinity column chromatography Huperzia serrata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminoethylpyridine + H2O + O2
-
 Huperzia serrata pyridine-2-carbaldehyde + NH3 + H2O2
-
 ?
3-aminomethylpyridine + H2O + O2
-
 Huperzia serrata pyridine-3-carbaldehyde + NH3 + H2O2
-
 ?
4-aminomethylpyridine + H2O + O2
-
 Huperzia serrata pyridine-4-carbaldehyde + NH3 + H2O2
-
 ?
benzylamine + H2O + O2
-
 Huperzia serrata benzaldehyde + NH3 + H2O2
-
 ?
cadaverine + H2O + O2 best substrate Huperzia serrata DELTA1-piperideine + NH3 + H2O2
-
 ?
histamine + H2O + O2
-
 Huperzia serrata (imidazol-4-yl)acetaldehyde + NH3 + H2O2
-
 ?
putrescine + H2O + O2
-
 Huperzia serrata ?
-
 ?
spermidine + H2O + O2
-
 Huperzia serrata ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 78000, SDS-PAGE Huperzia serrata
homodimer 2 * 77676, calculated from amino acid sequence Huperzia serrata

Synonyms

Synonyms Comment Organism
CAO
-
 Huperzia serrata
Copper amine oxidase
-
 Huperzia serrata

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
 cadaverine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
1.4
-
 benzylamine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
1.8
-
 histamine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
1.9
-
 putrescine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
1.9
-
 3-aminomethylpyridine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
2.3
-
 spermidine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
2.9
-
 2-aminoethylpyridine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
3.1
-
 4-aminomethylpyridine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 pH optimum for the piperideine-forming activity oft he enzyme Huperzia serrata

pH Range

pH Minimum pH Maximum Comment Organism
6.8 8.5 piperideine-forming activity Huperzia serrata

Cofactor

Cofactor Comment Organism Structure
2,4,5-trihydroxyphenylalanine quinone
-
 Huperzia serrata

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.6
-
 putrescine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
1.6
-
 4-aminomethylpyridine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
1.6
-
 3-aminomethylpyridine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
1.7
-
 2-aminoethylpyridine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
2.6
-
 histamine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
2.8
-
 benzylamine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
2.9
-
 spermidine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
3.3
-
 cadaverine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata